摘要
目的:建立玉叶金花的质量控制方法。方法:采集来自贵州和广东的13批次玉叶金花药材,采用显微鉴别方法鉴定玉叶金花叶表面特征和粉末特征,采用薄层色谱法对玉叶金花真实性进行鉴定;按照《中国药典》2015年版通则规定对玉叶金花水分、总灰分及醇溶性浸出物进行研究。结果:玉叶金花的显微特征明显,叶上表皮细胞呈类多角形、垂周壁增厚、气孔少见,叶下表皮细胞不规则形状、垂周壁波状弯曲、气孔为平轴式、非腺毛多见;粉末显微特征中非腺毛众多、表面光滑、导管多为具缘纹孔、偶见螺纹导管,石细胞众多、呈长方形、方形及类圆形;薄层色谱中主斑点清晰、分离好,斑点颜色和位置与对照药材一致;13批次玉叶金花的水分、总灰分及醇溶性浸出物存在差异。结论:本研究建立的玉叶金花的质量控制研究方法专属性强,稳定性和重复性良好,为该药材的真实性鉴定和质量评价提供了参考依据。
Objective:To establish a quality control method of Mussaenda pubescens.Methods:The microscopic identification method was applied to identify the surface characteristics of the leaves and powders of 13 batches of M.pubescens.The thin layer chromatography(TLC)was used to qualitatively identify the authenticity of M.pubescens.According to the general rules of the Chinese Pharmacopoeia(2015 edition),water content,total ash and alcohol soluble extract of M.pubescens were determined.Results:The leaf epidermal cells were usually irregular or polygonal in shape,and the stomata type was plane axis.Non-glandular hairs were found covering the leaf epidermis.A large number of bordered pit vessels were observed.In the thin-layer chromatography,the main spots were clear and the resolution was good.The spot color and position were consistent with the reference crude herb.The average moisture content,the total ash and the alcohol soluble extract of 13 batches of M.pubescens were different.Conclusion:The quality control method of M.pubescens is specific and reproducible,with very good stability and repeatablity,providing a reliable foundation for the assessment of M.pubescens.
作者
秦兰
刘亭
孙佳
陆苑
潘洁
王永林
李勇军
林昌虎
刘春花
QIN Lan;LIU Ting;SUN Jia;LU Yuan;PAN Jie;WANG Yonglin;Li Yongjun;LIN Changhu;LIU Chunhua(Engineering Research Center for the Development and Application of Ethnic Medicine and TCM,Ministry of Education&State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550004,Guizhou,China;School of Pharmacy,Guizhou Medical University,Guiyang 550025,Guizhou,China;Guizhou Provincial Key Laboratory of Pharmaceutics,Guizhou Medical University,Guiyang 550004,Guizhou,China)
出处
《贵州医科大学学报》
CAS
2020年第4期408-414,共7页
Journal of Guizhou Medical University
基金
国家自然科学基金项目(U1812403)
中央引导地方科技专项项目[黔科中引地(2018)4006]
贵州省科技计划项目[黔科合平台人才(2016)5613,黔科合平台人才(2016)5677,黔科合平台人才(2017)5718]。
关键词
色谱法
薄层
质量控制
玉叶金花
显微特征
一般检查
醇溶性浸出物
chromatography
thin layer(TLC)
quality control
Mussaenda pubescens
morphological character
general inspection
ethanol-soluble extractives
