摘要
为了探究杧果在UV-B辐射增强条件下的叶片光合生理变化特点,以自然光为对照,在田间人工模拟UV-B辐射增强处理‘台农1号’杧果成年树,设置24和96 k J·m-2·d-1两个UV-B辐射增强处理,观测了叶片光化学反应、碳同化关键酶活性及其基因表达水平等的变化。结果表明,96 k J·m-2·d-1增强处理导致树体株产和果实可溶性糖、糖酸比降低,而24 k J·m-2·d-1增强处理除维生素C显著高于对照外,与对照无显著差异;96 k J·mm-2·d-1增强处理的叶片叶绿素a/b值、净光合速率、气孔导度、蒸腾速率和胞间CO2浓度等多显著低于对照和24 k J·m-2·d-1增强处理,光合色素含量、希尔反应活力、光化学猝灭系数和Rubisco活性等均明显高于对照;24k J·m-2·d-1增强处理的叶片希尔反应活力和Rubisco活性等均显著高于对照,净光合速率、气孔导度、蒸腾速率、胞间CO2浓度和光合色素含量等多与对照无显著差异;3月5日和4月22日96 k J·mm-2·d-1增强处理抑制Rubisco大亚基(Rbc L)编码基因表达。可见,UV-B 96 k J·m-2·d-1增强处理能直接引起气孔限制,进而抑制光合作用,并引起减产和品质变劣。
In order to investigate the characteristics of photosynthesis changes of mango leaves under enhanced UV-B radiation,the treatment of artificial simulation of enhanced UV-B radiation(24 and 96 k J·m-2·d-1)were conducted with the adult trees of‘Tainong 1’mango in the field,and the photochemical reactions,activities of key enzyme in carbon assimilation and the expression of gene were observed.The results showed that,compared to the control(natural lighting),there was a decrease of tree yield and soluble sugar and sugar-acid ratio of fruits under the 96 k J·m-2·d-1 treatment and no significant changes with 24 k J·mm-2·d-1 tratment except that vitamin C was significantly higher than the control.Chlorophyll a/b value,net photosynthetic rate,stomatal conductance,transpiration rate and intercellular CO2 concentration in leaves treated by 96 k J·m-2·d-1 were significantly lower than the control and treated by 24 k J·m-2·d-1.The content of photosynthetic pigment,the vitality of Hill reaction,the quenching coefficient of photochemistry the activities of Rubisco were significantly higher than the control.The Hill activity and Rubisco activity treated by 24 k J·m-2·d-1 were significantly higher than the control,net photosynthetic rate,stomatal conductance,transpiration rate,intercellular CO2 concentration and the content of photosynthetic pigment showed no significant difference compared to the control.The expression of coding gene of Rubisco big subunit(Rbc L)could be inhibited by the 96 k J·m-2·d-1 treatment 5th March and 22nd April.It is clear that stomatal limitation could be induced by 96 k J·m-2·d-1 treatment directly,and then photosynthesis would be inhibited and then result in the reduction of yield and the deterioration of quality.
作者
王红
岳堃
杨成坤
郭钰柬
王珮璇
周开兵
WANG Hong;YUE Kun;YANG Chengkun;GUO Yujian;WANG Peixuan;ZHOU Kaibing(Tropical Crop New Variety Breeding Education Engineering Center,Hainan University,Haikou 570228,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2020年第2期242-252,共11页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31460498)。
