摘要
以药食同源食品金银花为原料,采用水溶-醇沉方法结合高压液相制备,优化目标成分绿原酸萃取、分离条件,制备了绿原酸标准对照物质并进行分析鉴定。采用红外光谱、紫外光谱、质谱和核磁H谱、C谱对获得的标准物质进行化学结构鉴定,以高效液相色谱配紫外检测器对获得的标准物质进行均匀性(瓶间、瓶内)和不同温度下的短期(1周,常温,4℃,-20℃)、长期(6个月,-20℃)稳定性检验;采用同一方法多实验室联合定值方式进行定值分析。结果表明,研制的绿原酸标准物质纯度定值结果在99%以上,有效期至少6个月(-20℃)。结果表明,所制备的绿原酸标准物质纯度高,并具有良好的均匀性和稳定性,可用于金银花中绿原酸分析方法的确证和评价。
The chlorogenic acid reference material was prepared using honeysuckle as raw material by the method of water extraction and ethanol precipitation combined with medium pressure preparative liquid chromatography and further analyzed and identified.Firstly,the identification on chemical structure of the obtained reference material was carried out by FT-IR spectroscopy,UV spectroscopy,MS,NMR H and C spectra.Furthermore,the homogeneity(inter-and intra-bottle difference)and short-term(one week,room temperature,4℃,-20℃)and long-term(six months,-20℃)stability of the obtained standard reference material were evaluated using HPLC with UV detector.Finally,the multi-labjoint determination method was used to certificate the reference substance and the uncertainty of the certification results was evaluated.The obtained experimental results show that the purity value of the prepared chlorogenic acid is above 99%,and the effective period is at least 6 months(-20℃).This reference chlorogenic acid has high purity,good homogeneity and stability,and can be used for confirming and evaluating the analysis method of chlorogenic acid in the honeysuckle.
作者
潘明飞
杨晶莹
李睿
刘凯欣
王硕
Pan Mingfei;Yang Jingying;Li Rui;Liu Kaixin;Wang Shuo(State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science and Technology,College of Food Science and Engineering,Tianjin 300457)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2020年第4期224-232,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
国家重点研发计划项目(2017YFF0211002)。
关键词
金银花
绿原酸标准物质
结构鉴定
定值评价
honeysuckle
chlorogenic acid reference material
structure identification
evaluation
