摘要
目的运用网络药理学的方法探讨黄芪治疗重症肌无力(myasthenia gravis,MG)的潜在作用机制。方法利用中药系统药理学数据库与分析平台(TCMSP)和文献挖掘获取黄芪的活性成分及与活性成分相关的潜在靶点,利用人类基因数据库GeneCards搜索重症肌无力的疾病靶点,并采用Cytoscape3.7.1软件构建黄芪活性成分-疾病靶点网络;使用String数据库进行蛋白质-蛋白质相互作用分析,绘制蛋白相互作用网络(PPI);借助DAVID6.8数据库对黄芪治疗重症肌无力潜在作用靶点进行基因本体(GO)分析和基因组百科全书(KEGG)通路富集分析,以探究黄芪治疗重症肌无力的作用机制。结果筛选出黄芪20个活性成分,涉及12个作用靶点。GO分析结果显示,黄芪治疗重症肌无力的潜在基因的生物过程主要涉及神经递质结合、细胞外基质结合、核受体活性、转录因子活性、铵离子结合、类固醇受体激素结合、受体调节活性、生长因子受体结合等;KEGG通路富集分析结果显示,黄芪治疗重症肌无力潜在基因的通路主要涉及PI3K-Akt信号通路、AGE-RAGE信号通路、胆碱能突触通路、乙肝通路、EB病毒感染通路、EGFR酪氨酸激酶抑制剂耐药、HIF-1信号通路、大肠癌通路、雌激素信号通路、JAK-STAT等信号通路。结论黄芪治疗重症肌无力是多成分-多靶点-多途径的复杂过程,主要通过调控ESR1、IL-6、MYC、VEGFA、CRP、ESR2等靶点、PI3K-Akt信号通路、AGE-RAGE信号通路、EB病毒感染信号通路等多个途径发挥治疗重症肌无力的作用。
Objective To investigate the potential mechanism of Astragali Radix in the treatment of myasthenia gravis(MG)based on network pharmacology.Methods The main active ingredients of Astragali Radix were obtained through TCM System Pharmacology database and analysis platform(TCMSP)and literature exploration.The human gene database GeneCards was used to search for disease targets of MG.The Cytoscape 3.7.1 software was used to construct the active ingredient-disease target network of Astragali Radix.The String database was used to analyze protein-protein interaction and draw PPI.With the help of DAVID6.8 database,the potential target of Astragali Radix for MG was analyzed by gene ontology(GO)and genomic encyclopedia(KEGG)pathway enrichment.Results 20 active components of Astragali Radix were screened,involving 12 targets.GO analysis showed that the biological function of Astragali Radix in the treatment of MG is mainly related to the influence of neurotransmitter binding,extracellular matrix binding,nuclear receptor activity,transcription factor activity,ammonium ion binding,steroid hormone receptor activity steroid binding,receptor regulator activity,growth factor receptor binding,etc.The results of KEGG pathway enrichment analysis showed that the potential gene pathways of Astragali Radix in the treatment of MG mainly involve PI3K-Akt pathway,AGE-RAGE pathway,cholinergic synapse pathway,hepatitis B pathway,Epstein-Barr virus infection pathway,EGFR tyrosine kinase inhibitor resistance,HIF-1 pathway,Estrogen pathway,JAK-STAT pathway,etc.Conclusion Astragali Radix treatment of MG is a complex process of multi-component,multi-target and multi-channel.It mainly plays a role in the treatment of MG by regulating ESR1,IL-6,Myc,VEGFA,CRP,ESR2 and other targets,as well as PI3K Akt signal pathway,age-range signal pathway,EB virus infection signal pathway and other channels.
作者
梁玉华
李亦聪
邓太平
伍丽芳
LIANG Yuhua;LI Yicong;DENG Taiping;WU Lifang(Huizhou Municipal Central People′s Hospital,Huizhou 516001,China;Boluo County Traditional Chinese Medicine Hospital,Boluo 516000,China)
出处
《广东药科大学学报》
CAS
2020年第2期242-248,共7页
Journal of Guangdong Pharmaceutical University
基金
广东省中医药管理局"建设中医药强省"科研课题(20152085)。
关键词
网络药理学
黄芪
重症肌无力
分子机制
信号通路
network pharmacology
Astragali Radix
myasthenia gravis
molecular mechanism
signaling pathway
