摘要
油藏中普遍栖息着多种微生物,其生长代谢可在多个层面影响油田开发过程,但有关微生物多样性及群落组成的认知仍十分有限.采用基于16S r RNA基因的高通量测序技术解析了我国西北地区某油田10口油井采出水中微生物多样性与群落结构.采出水中变形菌门为优势类群,其下属的γ-变形菌纲、α-变形菌纲、ε-变形菌纲在多数样品中的相对丰度较高,而β-变形菌纲相对丰度较低(≤3.2%).假单胞菌属(Pseudomonas)、嗜冷杆菌属(Psychrobacter)和不动杆菌属(Acinetobacter)是相对优势的属,各采出井间微生物多样性与群落结构差别明显.共检出10个属的潜在硫化氢产生菌,硫酸盐还原菌的相对丰度低;硫磺单胞菌属(Sulfurospirillum)在所有样品中均存在,其相对丰度在0.02%~0.89%间波动,该类群在油田酸败中的作用有待研究.
Diverse groups of microorganisms reside in oil reservoir and these microbes can fundamentally affect the oil recovery process. However,knowledge about the microbial diversity and community structure in oilfield environment is limited. Adopting high-throughput sequencing technique,we disclosed the microbial diversity and composition in the produced water collected from 10 producing wells of an oilfield in Northwest China. Results showed that the Proteobacteria was dominant in produced water samples. The γ-,α-,and ε-proteobacteria were detected abundant in most samples,while the relative abundance of β-proteobacteria was less than 3.2%. Pseudomonas,Psychrobacter,and Acinetobacter were the abundant genera. Distinct microbial structure was observed among different producing wells.A total of 10 potentially sulfide-producing genera were detected,and the relative abundance of sulfate-reducing bacteria was low;by contrast,Sulfurospirillum was distributed in all samples,with the relative abundance ranging from 0.02% to 0.89%. The contribution of these sulfide-producing microorganisms to the souring of petroleum reservoirs needs to be further investigated.
作者
魏宠
王堑傧
杨春璐
史荣久
张颖
WEI Chong;WANG Qianbin;YANG Chunlu;SHI Rongjiu;ZHANG Ying(School of Environmental,Liaoning University,Shenyang 110036,China;Shenyang Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China;Key Laboratory of Pollution Ecology and Environment Engineering,Chinese Academy of Sciences,Shenyang 110016,China)
出处
《河南科学》
2020年第3期411-416,共6页
Henan Science
基金
国家自然科学基金资助项目(31570121)。
关键词
油田
细菌群落结构
高通量测序
采出水
硫化氢
oilfield
microbial community structure
high-throughput sequencing
produced water
hydrogen sulfide
