摘要
目的探讨脊髓损伤患者肠道菌群属水平变化特征及其在临床康复中的意义。方法 2018年1月至12月,纳入脊髓损伤患者23例(患者组)和健康受试者21例(对照组),采集粪便样本,应用16S rDNA高通量测序检测肠道菌群,并采用物种组成分析及随机森林机器学习等生物信息学方法分析两组间属水平的菌群分布及差异。结果患者组丰度升高的重要标志性菌属分别是:瘤胃菌科的UBA1819、 Ruminiclostridium 9、Ruminococcaceae NK4A214 group、 Ruminococcus 2、 Ruminococcaceae UCG-005、 Ruminiclostridium 5、Flavonifractor;理研菌科的Alistipes、 dgA-11 gut group、 Rikenellaceae RC9 gut group;毛螺菌科的[Eubacterium] oxidoreducens group;消化链球菌科的Intestinibacter;肠杆菌科的Escherichia-Shigella (志贺菌属);以及Tannerellaceae的Parabacteroides (|U|> 1.962, P <0.05)。患者组丰度降低的是梭杆菌科Fusobacterium菌属(|U|=-2.284, P <0.05)。结论脊髓损伤患者肠道菌群与健康对照组存在差异。脊髓损伤患者与抑郁相关的瘤胃菌科、与中枢神经系统疾病关系密切的瘤胃球菌,以及肠道内致病菌消化链球菌属、志贺菌属等的相对丰度增加;对肠道有益的产丁酸盐菌和抗炎性菌属的相对丰度降低。这些菌群变化可能有一定的临床意义。
Objective To investigate the changes of genus-level gut microbiome in patients with spinal cord injury and its significance in clinical rehabilitation.Methods Fecal samples were collected from 23 patients with spinal cord injury(patients group)and 21 healthy volunteers(control group).Gut microbiome was detected by 16S rDNA high-throughput sequencing.Bioinformatics methods such as species composition analysis and Random Forest were used to analyze the distribution and difference of genus-level gut microbiome between two groups.Results Compared with the control group,the increased important marker genera in the patients group were as follows:UBA1819,Ruminiclostridium 9,Ruminococcaceae NK4A214 group,Ruminococcus 2,Ruminococceae UCG-005,Ruminiclostridium 5,Flavonifractor belonging to Ruminococceae;Aglistes,dgA-11 gut group,Rikenaceae RC9 gut group belonging to Rikenellaceae;[Eubacterium]oxidoreducens group belonging to Lachnospiraceae;Intestinibacter belonging to Peptostreptococcaceae;Escherichia-Shigella belonging to Enterobacteriaceae;Tannerellaceae belonging to Parabacteroides(|U|>1.962,P<0.05).The decreased marker genera in the patients group was Fusobacterium of Fusobacteriaceae(|U|=-2.284,P<0.05).Conclusion There are significant differences of gut microbiome in spinal cord injury patients.The relative abundance of Ruminococcaceae relating to depression,Ruminococcus relating to central nervous system diseases,and enteropathogenic bacteria such as Escherichia-Shigella and Erysipelothrix increase;and the relative abundance of butyrate-producing bacteria and anti-inflammatory bacteria benefitting to the intestine decrease;which may play a role in clinic.
作者
汪俊宇
庞日朝
王文春
王美玉
向武
赵小华
张安仁
WANG Jun-yu;PANG Ri-zhao;WANG Wen-chun;WANG Mei-yu;XIANG Wu;ZHAO Xiao-hua;ZHANG Anren(Department of Rehabilitation Medicine,the General Hospital of Western Theater Command,Chengdu,Sichuan 610083,China;State Key Laboratory of Biotherapy of Sichuan University,Chengdu,Sichuan 610041,China;Tianfu College,Southwestern University of Finance and Economics,Chengdu,Sichuan 610052,China)
出处
《中国康复理论与实践》
CSCD
北大核心
2020年第4期393-399,共7页
Chinese Journal of Rehabilitation Theory and Practice
基金
国家自然科学基金面上项目(No.81674044,No.81973927)
四川省卫计委普及应用项目(No.18PJ509)
四川省医学会青年创新科研课题(No.Q17007)。
关键词
脊髓损伤
肠道菌群
临床康复
spinal cord injury
gut microbiome
clinical rehabilitation
