摘要
研究了太子参须多糖粗提物对RAW264.7巨噬细胞体外增殖及细胞免疫活性的影响,进一步评价了太子参须多糖对隐球菌感染模型小鼠的免疫调理作用.结果表明,太子参须多糖粗提物能显著提高RAW264.7巨噬细胞的相对增殖率,促进巨噬细胞对中性红和隐球菌的吞噬能力;太子参须多糖能恢复隐球菌感染小鼠的体质量,增加IL-10,IL-1β及TNF-α的分泌,降低IL-6的分泌,减少感染小鼠肺部和脑部的隐球菌数量,并增强了肺部TLR4受体、 CREB和p-CREB的表达.因此得出,太子参须多糖能通过提高巨噬细胞的免疫活性调节小鼠免疫功能,从而增强机体的抗炎能力.
In this study, the effects of crude polysaccharides from Radix pseudostellariae on the proliferation and cellular immune activity of RAW264.7 macrophages were investigated, and the immunoregulation of R. pseudostellariae polysaccharides on Cryptococcus neoformans-infected mice was evaluated. The results indicated that the crude polysaccharides from R. pseudostellariae could significantly increase the relative proliferation rate of RAW264.7 macrophages and promote their phagocytic ability to neutral red and Cryptococcus. In addition, the polysaccharides of R. pseudostellariae could partly ameliorate the body mass of mice infected with Cryptococcus, elevate the levels of IL-10, IL-1β and TNF-α, and decrease the levels of IL-6 in the serum. In the polysaccharide-treated group the number of Cryptococcus in the lungs and the brain was reduced, and the protein levels of TLR4 receptor, CREB and p-CREB in the lung were enhanced. It was concluded that the crude polysaccharides of R. pseudostellariae can improve the immune function and enhance anti-inflammation ability in mice possibly via the immunological activity modulations of the macrophages.
作者
陈小英
曾晏萍
刘汉儒
袁吕江
刘雪梅
CHEN Xiao-ying;ZENG Yan-ping;LIU Han-ru;YUAN Lyu-jiang;LIU Xue-mei(School of Pharmaceutical Sciences,Southwest University,Chongqing 400715,China)
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第4期56-64,共9页
Journal of Southwest University(Natural Science Edition)
基金
重庆市重点产业共性关键技术创新专项(cstc2016zdcy-ztzx10001)
中央高校基本科研业务费专项(XDJK2018B037,XDJK2018D027)。
关键词
太子参须
多糖提取物
RAW264.7
隐球菌
免疫功能
Radix pseudostellariae
polysaccharide extract
RAW264.7
Cryptococcus neoformans
immune function
