摘要
[目的]对不同产地的北柴胡和南柴胡的总皂苷及4种单体成分(柴胡皂苷a、d、c、f)进行含量测定。[方法]使用柴胡皂苷d作为对照品,采用紫外可见分光光度法,在536 nm处测定24批北柴胡和5批南柴胡样品中的总皂苷的含量。使用HPLC-ELSD方法对柴胡样品中的柴胡皂苷a、d、c、f进行含量测定。[结果]分析得出,同一样品中柴胡皂苷d的含量要普遍高于柴胡皂苷a的含量,不同产地的柴胡中柴胡皂苷a、d、c、f的含量之间具有显著性差异。柴胡总皂苷在0.08~0.40 mg之间具有良好的线性关系(r=0.999)。加样回收率在99.2%~103%之间,RSD=1.42%。不同产地的南北柴胡总皂苷含量在5.32%~13.9%之间,其中南柴胡的总皂苷含量要高于北柴胡的含量,其中产自山东临沂的南柴胡含量最高,达到了13.9%。[结论]柴胡的质量评价不能只测定其中柴胡皂苷a的含量,应该以总皂苷为主,同时对4种柴胡皂苷的含量进行测定才更为准确。
[Objective]To determine total saponins and four monomers saponins(saikosaponin a,d,c and f)in Bupleuri Radix from different habitats.[Methods]Using saikosaponin d as the standard,the total saponins in 24 batches of B.chinense DC.and 5 batches of B.scorzonerifolium Willd.were determined at 536 nm by UV-Vis spectrophotometry.The content of saikosaponin a,d,c and f were determined by HPLC-ELSD.[Results]The content of saikosaponin d in the same batch of samples was generally higher than that of saikosaponin a.There were significant differences in the contents of saikosaponin a,c,d and f from different habitats.There was a good linear relationship in range of 0.08~0.4 mg(r=0.999).The recovery of sample addition ranged from 99.2%to 103%,RSD=1.42%.The content of total saponins ranged from 5.32%to 13.9%,and the content of total saponins in B.scorzonerifolium Willd.was higher than that in B.chinense DC.The content of total saponins in B.scorzonerifolium Willd.from Linyi(Shandong Province)was the highest,reaching 13.9%.[Conclusion]The quality evaluation of Bupleuri Radix can not only determine the content of saikosaponin a,it should determine saikosaponins and saikosaponin a,c,d and f at the same time for more accurately.
作者
郭泰麟
康廷国
张慧
GUO Tailin;KANG Tingguo;ZHANG hui(College of pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116001,China)
出处
《天津中医药大学学报》
CAS
2020年第2期221-225,共5页
Journal of Tianjin University of Traditional Chinese Medicine
基金
辽宁省教育厅高校优秀科技人才支持计划(LR2015042)。
关键词
柴胡
狭叶柴胡
柴胡皂苷A
柴胡皂苷c
柴胡皂苷D
柴胡皂苷f
柴胡总皂苷
含量测定
Bupleurum chinense DC
Bupleurum scorzonerifolium Willd
saikosaponin a
saikosaponin c
saikosaponin d
saikosaponin f
saikosaponins
content determination
