摘要
目的:选择7种有效成分为指标,对内蒙古地区不同产地的黄芪药材进行品质的初步评价。方法:采用高效液相色谱法对毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素4种黄酮类成分进行含量测定,采用高效液相色谱联用蒸发光检测器法对黄芪甲苷、黄芪皂苷Ⅰ、黄芪皂苷Ⅱ3种皂苷类成分进行含量测定,根据测得的40个产地黄芪样品中各指标成分含量,应用SPSS并以组间联结作为聚类方法,对不同产地黄芪样品进行聚类分析。结果:黄酮类、皂苷类成分含量测定方法均符合方法学考察要求,毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素、黄芪皂苷Ⅰ、黄芪皂苷Ⅱ、黄芪甲苷的质量分数分别为0.0216%~0.1503%、0.0026%~0.0602%、0.0002%~0.0125%、0.0003%~0.0135%、0.0239%~2.1890%、0.0413%~0.3911%、0.0407%~0.1065%,聚类分析结果将不同产地黄芪样品分为3类,生长于阴山山脉山区的样品聚为一类,生长于阴山山脉山下周边农区的样品聚为一类,不在阴山山脉范围内的样品聚为一类。结论:本研究所采用的含量测定方法简单、稳定、准确,适用于黄芪中黄酮类、皂苷类成分的测定;生长在阴山山脉范围内的黄芪有效成分含量积累较多,品质较好,其中在包头市的固阳县与土默特右旗所产黄芪的品质最佳。
Objective:Seven effective components of Astragali Radix were selected as the index components to determine the content and compare the quality of Astragali Radix in different regions of Inner Mongolia.Methods:HPLC-UV was used to determine the contents of 4 flavonoids(calycosin-7-glucoside,ononin,calycosin and formononetin).HPLC-ELSD was used to determine the contents of 3 saponins(astragalosideⅣ,astragaloside I and astragalosideⅡ).The cluster analysis of Astragali Radix samples from different regions was carried out by using SPSS and using the cluster connection as the cluster method.Results:The determination methods of flavonoids and saponins were investigated by means of methodology,and the results conformed to the requirements.Content range of calycosin-7-glucoside,ononin,calycosin,formononetin,astragaloside I,astragalosideⅡand astragalosideⅣare 0.0216%-0.1503%,0.0026%-0.0602%,0.0002%-0.0125%,0.0003%-0.0135%,0.0239%-2.1890%,0.0413%-0.3911%and 0.0107%-0.1065%,respectively.The cluster analysis results divided Astragali Radix samples from different habitats into three categories:the samples in the Yin Mountains range were classified into one category;the samples surrounding agricultural areas under the Yin Mountains were classified into one category;and the samples not within the range of the Yin Mountains range were classified into one category.Conclusion:The method is simple,stable and accurate.It is suitable for the determination of flavonoids and saponins in Astragali Radix.Astragali Radix growing in the range of Yin Mountains had a large accumulation of effective components and Astragali Radix produced in Guyang County and Tumed Right Banner had the best quality.
作者
李紫岩
杨敏
王杰
张春红
李旻辉
LI Zi-yan;YANG Min;WANG Jie;ZHANG Chun-hong;LI Min-hui(Baotou Medical College,Baotou 014060,China;Inner Mongolia Autonomous Region Academy of Chinese Medicine,Hohhot 010110,China)
出处
《中国现代中药》
CAS
2020年第1期65-73,共9页
Modern Chinese Medicine
基金
内蒙古科技创新引导项目(KCBJ2018040)
内蒙古自治区科技成果转化项目(CGZH2018174)
内蒙古自治区科技计划项目(201701040)
公益性行业科研专项(201507002)
财政部中央本级专项(2060302)
国家中药材产业技术体系综合试验站专项基金(CARS-21)。
关键词
黄芪
黄酮
皂苷
含量测定
聚类分析
Astragali Radix
flavonoids
saponins
content determination
clustering analysis
