摘要
目的建立同时分析头发中5-MeO-DiPT等12种新型色胺类致幻剂的超高效液相色谱-串联质谱(UPLC-MS/MS)方法。方法以赛洛西宾-d4和赛洛新-d10为内标,20 mg头发样品加入1 mL提取液(含内标2 ng/mL的0.1%甲酸水溶液)后冷冻研磨,离心取上清液,经Waters Acquity^TM UPLC HSS T3色谱柱分离,采用多反应监测模式同时测定12种新型色胺类致幻剂。结果头发样品中12种色胺类新精神活性物质的检出限为1~10pg/mg,定量限为3~50pg/mg。在相应浓度范围内,12种色胺类新精神活性物质均具有良好的线性关系,相关系数大于0.99。本方法准确度为91.3%~113.5%,日间和日内精密度(RSD)均小于15%,回收率大于80%,无明显基质效应。结论该方法前处理简单、灵敏度高、选择性好,适用于法医毒物分析中新型色胺类致幻剂的鉴定。
Objective To development a method for simultaneous determination of 12 tryptamine hallucinogens in hair samples by UPLC-MS/MS.Methods With Psilocybin-d4 and Psilocin-d10 as internal standards,20 mg hair sample was frozen and ground after adding 1 ml extract,and centrifuged to obtain the supernatant.The supernatant was then separated in a waters AcquityTM UPLC HSS T3 column with a gradient elution,finally analyzed with multiple reaction monitoring(MRM)mode.Results The limits of detection(LOD)of 12 tryptamine-type new psychoactive substances in hair samples are ranging from 1-10 pg/mg,the lower limits of quantitation(LLOQ)are ranging of 3-50 pg/mg.In the corresponding concentration range,there was a good linearity for all analytes with correlation coefficients over 0.99.The accuracy was 91.3%-113.5%,the intra-day and inter-day precision were less than 15%,and the extraction recovery of all analytes were above 80%.Besides,no significant matrix effect was exhibited.Conclusion The established method is rapid,sensitive and specific,and it be used for the authentic cases of forensic toxicology.
作者
施妍
王柔嘉
强火生
沈保华
沈敏
刘伟
SHI Yan;WANG Roujia;QIANG Huosheng;SHEN Baohua;SHEN Min;LIU Wei(Academy of Forensic Science,Shanghai Key Laboratory of Forensic Medicine,Shanghai Forensic Science Platform,Academy of Forensic Science,Ministry of Justice,Shanghai 200063,China)
出处
《中国司法鉴定》
2020年第2期17-24,共8页
Chinese Journal of Forensic Sciences
基金
国家自然科学基金(81971789)
中央科研院所青年推进项目(2019G-4)
上海市法医学重点实验室项目(17DZ2273200)
上海市司法鉴定专业技术服务平台资助项目(16DZ2290900)
上海市科技攻关项目(19DZ1200600)
上海市法医学重点实验室开放课题(KF1908)。
