摘要
为了更好地发挥双歧杆菌的对人体健康的保健作用,以双歧杆菌和低聚果糖的混合溶液为芯材,海藻酸钠与乳清蛋白为壁材,采用内源乳化法制备双歧杆菌微胶囊。将湿胶囊的包埋率作为评价指标,通过单因素和响应面试验确定双歧杆菌微胶囊制备的最优条件。结果表明,当海藻酸钠质量分数为2%,乳清蛋白和海藻酸钠质量比为2∶1,碳酸钙和海藻酸钠质量比为1∶2,水相与油相体积比为2∶5,冰醋酸为400μL时,双歧杆菌湿润微胶囊包埋率可达到81.15%;将最佳工艺条件下得到的湿胶囊冷冻干燥制成干胶囊,进行耐胃酸、胆盐、肠道释放性和消化道体系实验,以游离菌作为对照组,结果发现干胶囊在经过模拟消化液实验后,每毫升活菌数均高于游离菌,同时具有良好的肠道释放性。由此表明,经过优化工艺条件制备的双歧杆菌微胶囊理化性能要优于游离菌。
The aim of this study was to better exert the effects of Bifidobacterium on human health.The mixed solution of Bifidobacterium and oligofructose was used as the core material,sodium alginate and whey protein were used as wall materials,and Bifidobacterium microcapsules were prepared by endogenous emulsification.The embedding rate of wet capsules was used as an evaluation index,and the optimal conditions for preparation of Bifidobacterium microcapsules were determined by single factor and response surface tests.The experiment results showed that when the concentration of sodium alginate was 2%,the mass ratio of whey protein to sodium alginate was 2∶1,the mass ratio of calcium carbonate to sodium alginate was 1∶2,the volume ratio of water phase to oil phase was 2∶5,and the glacial acetic acid was 400μL,the moment embedding rate of the Bifidobacterium wet microcapsules can reach a maximum of 81.15%.The wet capsules obtained under the optimal process conditions were freeze-dried to make dry capsules,and tested for tolerance to gastric acid and bile salt and intestinal release and digestive system,using the free bacteria as the control group.After the experiment of simulated digestive juice,it was found that the number of live bacteria per milliliter was higher than that of free bacteria.The result showed that,the physical and chemical properties of Bifidobacterium microcapsules prepared by optimized strip were better than that of free bacteria.
作者
李作美
许晓云
刘琦
LI Zuomei;XU Xiaoyun;LIU Qi(College of Food and Biotechnology Engineering,Bengbu University,Bengbu 233000,China;College of Life Sciences,University of Science and Technology of China,Hefei 230027,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2020年第6期155-162,共8页
Food and Fermentation Industries
基金
2019年高校优秀青年骨干人才国内访问研修项目(gxgnfx2019053)
2018年度安徽省省级自然科学研究重点项目(KJ2018A0570)
2016年安徽省质量工程项目大学生创客实验室建设计划(2016ckjh118)。
关键词
双歧杆菌
微胶囊
响应面
优化
理化性能
Bifidobacterium
microcapsule
optimization
response surface
physical and chemical properties
