摘要
在含深度共熔溶剂的非水相体系中,利用固定化Kurthia gibsonii SC0312细胞高效催化外消旋1-苯基-1,2-乙二醇(PED)不对称氧化以制备对映体纯的(S)-PED.在所考察的4种离子液体和2种深度共熔溶剂中,氯化胆碱/尿素([ChCl][U])对Kurthia gibsonii SC0312细胞表现出良好的生物相容性,能适当改变细胞膜的完整性,有效提高反应的催化效率.在[ChCl][U]体积分数为4%、缓冲液pH值为7.5、反应温度为35℃、细胞质量浓度为60 mg/mL、底物浓度为100 mmol/L的条件下,反应初速度为8.25 mmol/(L·h),反应仅7 h产率及(S)-PED e.e.值即分别达49.6%和99.9%.
Highly efficient asymmetric oxidation of racemic 1-phenyl-1,2-ethanediol(PED)was successfully conducted to prepare enantiomerically pure(S)-PED with immobilized Kurthia gibsonii SC0312 cells in non-aqueous system containing DESs.Among the 4 kind of tested ionic liquids and 2 kind of deep eutectic solvents,[ChCl][U]manifested better biocompatibility with Kurthia gibsonii SC0312 cells,and it can appropriately change the cell membrane integrity,thereby effectively improve the catalytic efficiency of this reaction.When[ChCl][U]content is 4%(volume fraction),buffer pH is 7.5,reaction temperature is 35℃,cell concentration is 60 mg/mL,and substrate concentration is 100 mmol/L,the initial reaction rate reaches 8.25 mmol/(L·h),and after 7 hours'reaction,the yield rate and(S)-PED e.e.value reach 49.6%and 99.9%,respectively.
作者
娄文勇
赵莹
彭飞
LOU Wenyong;ZHAO Ying;PENG Fei(School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China)
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2019年第11期62-70,共9页
Journal of South China University of Technology(Natural Science Edition)
基金
国家自然科学基金资助项目(21676104,21878105)。
