摘要
目的分析肺结核患者与健康对照之间外周血单个核细胞(peripheral blood mononuclear cell, PBMC)中长链非编码RNA(long non-coding RNA, lncRNA)MALAT1、NEAT1和NEAT2的表达差异。方法利用定量逆转录聚合酶链式反应技术检测79例肺结核患者和82例健康对照PBMC中lncRNA的表达水平,同时进一步分析lncRNA表达水平与肺结核患者部分临床特征、实验室指标之间的联系。结果肺结核患者PBMC中MALAT1、NEAT1的表达水平均高于健康对照,差异有统计学意义(Z=-4.386,P<0.001;Z=-10.175,P<0.001);NEAT2在两组之间的表达差异无统计学意义(Z=-0.203,P=0.839)。LncRNA表达水平与肺结核患者部分临床特征、实验指标关联分析结果显示,初治肺结核患者PBMC中NEAT2的表达水平高于复发肺结核患者,而痰涂片阳性肺结核患者PBMC中NEAT2的表达水平低于痰涂片阴性患者,差异均有统计学意义(均有P<0.05);MALAT1水平与血沉含量之间呈负相关(r_s=-0.256,P=0.034)。结论 MALAT1、NEAT1在肺结核患者外周血中是异常表达的,可能参与了肺结核的发病过程。
Objective To analyze the differences in the expression levels of the lncRNA MALAT1,NEAT,NEAT2 in peripheral blood mononuclear cell(PBMC)from tuberculosis patients and healthy controls.Methods We detected the lncRNA expression levels in PBMC from 79 tuberculosis patients and 82 healthy controls by quantitative reverse transcription polymerase chain reaction,and analyzed the correlation between lncRNA expression levels and some clinical features and laboratory indicators in tuberculosis patients.Results The expression levels of MALAT1,NEAT1 in PBMC of tuberculosis patients were significantly higher than healthy controls(Z=-4.386,P<0.001;Z=-10.175,P<0.001).There was no significant difference in the expression of NEAT2 between tuberculosis patients and healthy controls(Z=-0.203,P=0.839).The correlation results of lncRNA levels and some clinical features,laboratory indicators in tuberculosis patients suggested that the NEAT2 level in PBMC of newly treated tuberculosis patients was higher than recurrent tuberculosis patients,while the NEAT2 level in PBMC of sputum smear positive tuberculosis patients was lower than that of sputum smear negative tuberculosis patients(all P<0.05).There was a negative correlation between MALAT1 level and erythrocyte sedimentation rate(r_s=-0.256,P=0.034).Conclusion MALAT1 and NEAT1 are abnormally expressed in PBMC of tuberculosis patients,and may be involved in the pathogenesis of pulmonary tuberculosis.
作者
厉虹淼
陈双双
包训迪
张根友
史四九
刘晓宁
章鑫丽
刘双
王华
李晔
LI Hong-miao;CHEN Shuang-shuang;BAO Xun-di;ZHANG Gen-you;SHI Si-jiu;LIU Xiao-ning;ZHANG Xin-li;LIU Shuang;WANG Hua;LI Ye(BCG vaccine division n Anhui Institute of Tuberculosis Control,Hefei 230022,China;Science and Education Division,Anhui Institute of Tuberculosis Control,Hefei 230022,China;Anhui Province tuberculosis Rtjererue Libor atory,Htfei 230022,China;Tuberculosis Department,Anhui Provincial Chest Hospital,Hefei 230022,China)
出处
《中华疾病控制杂志》
CAS
CSCD
北大核心
2020年第2期155-159,共5页
Chinese Journal of Disease Control & Prevention
基金
安徽省自然科学基金项目(1908085QH368)。
