摘要
半胱天冬氨酸酶(metacaspases,MC)在植物生长发育调控和逆境胁迫的细胞程序性死亡(PCD)中扮演着重要角色。本研究对香蕉MuMCs基因序列和蛋白序列进行生物学信息分析,采用qTR-PCR技术对香蕉幼苗中MuMCs进行基因表达分析。结果表明,香蕉MuMCs具有8个成员,分为2种类型,Ⅰ型MuMC1~MuMC5和Ⅱ型MuMC6~MuMC8;顺式调控元件预测显示在MuMCs启动子区域存在19种激素和逆境胁迫相关的顺式调控元件;基因表达分析发现Mu MCs具有组织表达特性,在大部分MuMCs中叶的相对表达量最高,假茎的次之,根的最低;此外,Mu MCs对盐胁迫有不同程度的应答模式,盐胁迫后MuMC5和MuMC8相对表达量最高,MuMC2和MuMC6相对表达量最低。本研究为深入了解MuMCs基因特点及验证其生物学功能提供了理论基础,同时也为进一步探究PCD的调控途径提供了理论依据。
Metacaspases play critical roles in developmentally regulated and stress induced programmed cell death(PCD).In this study,the gene sequence and protein sequence of Mu MCs in banana were analyzed by bioinformatics,and the expression analysis of MuMCs in banana seedlings were revealed by qTR-PCR technology.The results showed that eight members of Mu MCs in banana were divided into 2 types:MuMC1~MuMC5 belong to typeⅠand typeⅡinclude MuMC6~MuMC8.Cis-regulatory element prediction showed that there were 19 species hormone and stress-related cis-regulatory elements in MuMCs promoter regions.Expression analysis showed that MuMCs have tissue specific expression properties,and the relative expression of the middle leaves of most MuMCs was the highest,followed by the pseudostems and the roots.Mu MCs have varying degrees of response to salt stress.The relative expression of MuMC5 and MuMC8 was the highest,and the relative expression of MuMC2 and MuMC6 was the lowest.This study provided insights into the characteristics of MuMC genes and laid theoretical basis for further verification of the biological functions of Mu MCs and the regulation of PCD.
作者
唐露
孙梦利
杨振
乔飞
李新国
Tang Lu;Sun Mengli;Yang Zhen;Qiao Fei;Li Xinguo(College of Horticulture and Landscape Architecture of Hainan University,Haikou,570228;Tropical Crops Genetic Resources Institute,Chinese A-cademy ofTropical Agricultural Sciences,Danzhou,571737)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2020年第1期181-194,共14页
Genomics and Applied Biology
基金
国家自然科学基金项目(31760549)资助。
