摘要
β-淀粉酶(BAM)在植物体内参与多种生物学过程的调控,并能对激素与非生物胁迫等多种外界刺激做出响应。为了解玉米BAM家族的特征和表达模式,利用生物信息学方法,对玉米BAM基因家族成员进行了全基因组鉴定,并分析了其编码蛋白、染色体定位、基因结构、顺式作用元件、共线性、组织表达特异性以及模拟雹灾和施用褪黑素处理后基因的表达模式。结果表明,在玉米中鉴定出16个ZmBAM成员且均具有典型的Glyco_hydro_14结构域,且大多数为亲水蛋白。系统发育分析显示,ZmBAM蛋白可分为3个类群,相同类群具有相似的基因结构及基序分布。顺式作用元件分析表明,大部分ZmBAM基因的表达可能与植物激素、非生物胁迫和光反应等相关。共线性分析结果显示,玉米与拟南芥和大豆BAM基因家族有一定的同源性,与水稻亲缘关系更近。qRT-PCR分析发现,在模拟雹灾胁迫下,大部分基因呈上调趋势,施用褪黑素后显著上调,为进一步揭示玉米BAM基因家族在响应非生物胁迫中的功能提供了参考依据。
β-Amylase(BAM)is involved in regulating various biological processes in plants and responds to multiple external stimuli such as hormones and abiotic stress.To explore the characteristics and expression patterns of the BAM gene family in maize,we performed a genome-wide identification of the maize BAM gene family using bioinformatics methods.We also analyzed the encoded proteins,chromosomal localization,gene structure,cis-acting elements,synteny analysis,tissue-specific expression,and the expression patterns of the genes after simulated hail stress and melatonin treatment.The results showed that 16 ZmBAM members were identified in maize,all of which contained the typical Glyco_hydro_14 domain,with most of them being hydrophilic proteins.Phylogenetic analysis indicated that ZmBAM protein can be divided into three groups,with genes in the same group sharing similar gene structures and motif distributions.Cis-acting element analysis suggested that the expression of most ZmBAM genes might be related to plant hormones,abiotic stress,and light responses.Synteny analysis revealed a certain level of homology between the maize BAM gene family and those of Arabidopsis and soybean,with a closer relationship to rice.qRT-PCR analysis showed that,under simulated hail stress,most genes were upregulated,and their expression was significantly upregulated after melatonin treatment.This study provided a reference for further understanding the function of the maize BAM gene family in response to abiotic stress.
作者
蒋傲男
陈许双
杨启帆
刘广周
崔彦宏
边大红
JIANG Aonan;CHEN Xushuang;YANG Qifan;LIU Guangzhou;CUI Yanhong;BIAN Dahong(College of Agronomy,Hebei Agricultural University,Baoding 071000,China)
出处
《华北农学报》
北大核心
2025年第6期50-61,共12页
Acta Agriculturae Boreali-Sinica
基金
“十四五”国家重点研发计划项目(2023YFD2301502)
河北省玉米产业技术体系(HBCT2023020101)。
