摘要
目的采用网络药理学方法探讨失笑散的功效物质基础对子宫内膜异位症痛经方面的作用机制。方法依托TCMSP数据库检索失笑散中五灵脂,BATMAN-TCM数据库检索失笑散中蒲黄相关的所有化学成分,整合作用靶点预测(STPD),进而构建化合物-靶点网络图;通过Gene Cards、DisGeNET数据库筛选子宫内膜异位症痛经相关的靶点,进而构建疾病靶点相互作用网络图;筛选药物靶点和疾病靶点相互作用图的核心靶点。采用DAVID数据库对失笑散作用靶点进行生物功能及代谢通路分析。结果选择筛选出失笑散中的15个活性成分,共涉及225个作用靶点;通过网络拓扑特征评价筛选出与失笑散在子宫内膜异位症痛经方面作用的核心靶点41个,主要通过癌症通路、粘着斑、丝裂原活化蛋白激酶、细胞凋亡、NOD样受体、T细胞受体信号通路发挥对子宫内膜异位症痛经的治疗作用。结论通过网络药理学验证了失笑散多成分、多靶点、整体调节的作用特点,预测了失笑散在子宫内膜异位症痛经中的主要作用机制,为其活性成分研究和实验研究提供理论依据。
Objective Network pharmacology method was adopted in this study to explore the active compounds and mechanism of Shixiao Powder for angiogenesis. Methods Chemical components and targets related to the four traditional Chinese medicine(TCM) herbs were searched through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) and BATMAN-TCM,and then integrating target prediction database(STPD). Compound-target network were constructed to explore the mechanism of Shixiao Powder. Through DisGeNET database and Gene database, the targets of endometriosis dysmenorrhea were selected, and then the network diagram of disease-target interaction was constructed;The nuclear target of drug target and disease target interaction diagram was screened, DAVID was used to carry out GO analysis of the nuclear target, and KEGG database was used to carry on the related pathway enrichment of the nuclear target. Results Selecting the oral bioavailability(OB) ≥ 30%and drug likeness(DL) ≥ 0.18 as filter condition, 15 active ingredients and 225 corresponding protein targets were screened from Shixiao Powder. Through the evaluation of network topological characteristics, such as degree, medium degree, and proximity to the center, 41 targets were selected to serve as the nuclear target in atherosclerosis. The KEGG database was used for the enrichment of the selected targets, including Cancer pathway, focal adhesion, mitogenactivated protein kinase, apoptosis, NOD-like receptor, T-cell receptor signaling pathway which were screened which had effect on endometriosis dysmenorrhea. Conclusion This study revealed the main active compounds and possible mechanism of Shixiao Powder for treatment of angiogenesis and verified the characteristics of multi-components, multitargets, and integral regulation for Shixiao Powder, predicting the main possible mechanism for the treatment of endometriosis dysmenorrhea, which provided theoretical basis for the study of active ingredients and experimental research.
作者
伍娟娟
何嘉
邓海婷
刘秀芳
刘锐
Wu Juan-juan;He Jia;Deng Hai-ting;Liu Xiu-fang;Liu Rui(Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530011,China;School of Graduate,Guangxi University of Chinese Medicine,Nanning 530011,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2019年第11期2386-2395,共10页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委员会地区科学基因项目(81860829):通过SDF-1/CXCR4轴对PF大鼠BMSCs迁移及归巢的影响
负责人:刘锐。
