摘要
目的:观察失笑散各溶剂提取物对氧化型低密度脂蛋白(oxidized low density lipoprotein,ox-LDL)诱导的人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)损伤的保护作用,探讨失笑散保护血管内皮细胞的有效提取物及其可能作用机制。方法:体外培养HUVECs,并制备失笑散石油醚、乙酸乙酯、甲醇提取物。采用ox-LDL诱导制备HUVECs损伤模型,并采用失笑散各溶剂提取物干预损伤细胞。采用MTT比色法测定各组细胞增殖情况,试剂盒检测各组细胞上清液中超氧化物歧化酶(superoxide orgotein dismutase,SOD)的活力、谷胱甘肽-过氧化物酶(glutathione peroxidase,GSH-px)活力、TBA法测定丙二醛(maleic dialdehyde assay,MDA)含量。结果:25 mg·L^(-1)ox-LDL可抑制血管内皮细胞活性。与模型组比较,失笑散石油醚提取物(25.00 mg·L^(-1),12.50 mg·L^(-1))及甲醇提取物(25.00 mg·L^(-1),12.50 mg·L^(-1))可显著提高细胞活力(P<0.01,P<0.05),显著降低MDA含量,提高SOD、GSH-px活性(P<0.01,P<0.05),且失笑散甲醇提取物在25 mg·L^(-1)质量浓度下,与失笑散石油醚提取物比较,在MDA含量、SOD活力的指标上表现出更有效的保护作用(P<0.05)。结论:失笑散石油醚及甲醇提取物可显著降低ox-LDL诱导的HUVECs损伤,为失笑散保护血管内皮细胞的有效提取物,其机制可能与其降低MDA含量和提高SOD、GSH-px活性有关,通过调控氧化损伤机制保护血管内皮细胞。
Objective ： To study the protective effects of solvent extracts of Shixiao Powder on oxidized low density lipoprotein （ ox- LDL） induced human umbilical vein endothelial cell （HUVECs） injury and its possible mechanism of action. Methods： HUVECs were cultured in vitro, and human umbilical vein endothelial cell injury model was induced by 25 mg · L-1 ox-LDL. The prolifera- tion and the vitality of superoxide orgotein dismutase （SOD） and glutathione peroxidase （GSH-px） in the supernatant of each group were determined by MTY colorimetry. , TBA method to determine the content of maleic dialdehyde （MDA）. Results ： 25 mg· L^-1 ox-LDL can inhibit the vascular endothelial cell activity. Compared with that of the model group, petroleum ether extract （ 25.00 mg · L^- 1,12.50 mg· L^-1 ） and methanol extract （25.00 mg· L^-1 ） significantly increased the cell viability （ P 〈 0.01,P 〈 O. 05 ）, significantly decreased MDA content, increased SOD, GSH-px activity （ P 〈 0.01, P 〈 O. 05 ）. At the dose of 25 mg · L-1, compared with the petroleum ether extract group, methanol extract showed more effective protection on the indicators of MDA content and SOD activity （P 〈0.05）. Conclusion ： The oil and methanol extract of Shixiao Powder can significantly reduce the ox-LDL-induced cell injury of HUVECs being an effective extract of vascular endothelial cells,which may be related to the re- duction of MDA content and the increase of SOD and GSH-px activities. Besides, it can protect vascular endothelial cells by regula- ting the mechanism of oxidative damage.
作者
高兰
王侃
包贝华
贾媛
张恒玉
戎立保
GAO Lan;WANG Kan;BAO Beihua;JIA Yuan;ZHANG Hengyu;RONG Libao(TCM Hospital of Jiangning District of Nanjing City,Nanjing Jiangsu China 211100;Nanjing University of Chinese Medicine,Nanjing Jiangsu China 210046)
出处
《中医学报》
CAS
2018年第9期1728-1732,共5页
Acta Chinese Medicine
基金
江苏省南京市江宁区2017年第一批科技发展计划项目(2017Db19)
关键词
失笑散
氧化型低密度脂蛋白
人脐静脉内皮细胞
氧化损伤
Shixiao Powder
oxidized low density lipoprotein
human umbilical vein endothelial cell
oxidative damage
