摘要
本研究以海南南渡江野生唇(Hemibarbus labeo)为材料,采用腹腔注射植物血球凝集素(PHA)、秋水仙素,肾细胞直接制片法,分析唇染色体核型和银染(Ag-NORs)。结果显示,唇的二倍体染色体数目为50,核型公式为2n=18m+12sm+12st+8t,其染色体臂数(NF)为80,未发现异型染色体,Ag-NORs出现在第5对和第11对同源染色体的短臂末端。亚科鱼类染色体进化综合分析结果表明,唇具有亚科鱼类的原始核型。本研究不仅补充了唇的细胞遗传学数据,为研究亚科/属鱼类的分类与进化提供了数据资料,也为唇的种质资源保护、种质改良等工作奠定了理论基础。
In this study,the karyotype and Ag-NORs of wild Hemibarbus labeo from Nandujiang River of Hainan were analyzed using the methods of intraperitoneal injection of phytohemagglutinin(PHA)and colchicine,and direct preparation of head kidney cells.Results showed that H.labeo had a diploid chromosome number of 50,the karyotype formula was 2n=18m+12sm+12st+8t,the number of chromosome arms(NF)was 80,no heterotypic chromosome was observed,and Ag-NORs were located on the ends of the short arms of pair No.5 and pair No.11.Comprehensive analyses of the chromosomal evolution in subfamily Gobioninae indicated that H.labeo had the primitive karyotype of Gobioninae fish.Our study will not only supplement the cytogenetic data of H.labeo and provide information on the taxonomy and evolution of the subfamily Gobioninae,genus Hemibarbus,but will also lay the theoretical foundation for germplasm protection and germplasm improvement of H.labeo.
作者
李安
李高俊
王世锋
柳淑芳
周永灿
申志新
薛献坵
蔡岩
LI An;LI Gaojun;WANG Shifeng;LIU Shufang;ZHOU Yongcan;SHEN Zhixin;XUE Xianqiu;CAI Yan(Ocean College,Hainan University,Key Laboratory of Tropical Aquatic Biotechnology of Hainan Province,Haikou 570228;Hainan Academy of Ocean and Fisheries Sciences,Haikou 570100;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071)
出处
《渔业科学进展》
CSCD
北大核心
2020年第2期95-102,共8页
Progress in Fishery Sciences
基金
农业基础性长期性科技工作(ZX08S1623)
中央级科研院所基本科研业务费专项(20603022018009)
海南省省属科研院所技术开发研究专项(R100030.304
R100263.3)共同资助。
关键词
唇
染色体
核型
银染
亚科
Hemibarbus labeo
Chromosome
Karyotype
Ag-NORs
Gobioninae
