摘要
【目的】植物的种子灭菌和生根培养是组织培养的重要步骤。目前已报道的用于甘蓝型油菜种子灭菌的次氯酸钠(NaClO)浓度、灭菌时间和生根培养基的植物生长调节剂种类及其浓度均存在较大的差异,因此,本研究旨在筛选出甘蓝型油菜种子的最佳NaClO灭菌处理方法和生根培养基最适的植物生长调节剂及其浓度。【方法】比较了甘蓝型油菜的种子经不同浓度(浓度范围为0.1%~30.0%)NaClO灭菌后的发芽率、健康芽率和菌落率,筛选出最适的种子灭菌NaClO浓度,再在该最适NaClO浓度基础上找出了最适的种子灭菌时间;通过比较不同质量浓度(0.1~2.0 mg·L^−1)的植物生长调节剂萘乙酸(NAA)或吲哚丁酸(IBA)对甘蓝型油菜再生根的促进作用,找出油菜生根培养最适的植物生长调节剂种类及其浓度。【结果】甘蓝型油菜种子的最佳灭菌NaClO浓度为2.0%或3.0%,在3.0%NaClO下的最适灭菌时间为15 min,种子经该灭菌方法处理后的发芽率和健康芽率分别可达97.4%和85.9%,且菌落发生率最少。该油菜种子灭菌的方法也适用于其他9个植物品种,尤其适合于甘蓝型油菜、大白菜和拟南芥的种子灭菌。在生根培养基中添加0.1 mg·L^−1 NAA对甘蓝型油菜再生根的促进作用最强,油菜培养7 d后即有92.5%的累积生根率,再生根从切点处生长,且再生的须根数最多。【结论】本研究优化了甘蓝型油菜种子的NaClO灭菌方法和生根培养基的植物生长调节剂及其浓度,为高效的油菜组织培养研究奠定了基础。
【Objective】Seed sterilization and rooting culture are important steps in plant tissue culture.At present,there is considerable variation in the concentration of sodium hypochlorite(NaClO)and seed sterilization time of Brassica napus,as well as the type and concentration of hormone in the rooting media.The purpose of this study was to optimize the method of seed sterilization with NaClO and the formula of rooting medium for B.napus regeneration.【Method】The optimum concentration of NaClO for seed sterilization was screened by comparing the growth status(such as germination rate,healthy shoot rate and rate of colonies)of seeds treated with different concentrations(0.1%–30.0%)of NaClO,and the appropriate sterilization time was optimized based on the growth status of seeds treated with the optimal concentration of NaClO for 10—30 min.Moreover,the optimum formula of rooting media was screened by comparing the rooting regeneration of seedlings in the media containing 0.1–2.0 mg·L^−1 naphthylacetic acid(NAA)/indolebutyric acid(IBA).【Result】The optimum concentration of NaClO for seed sterilization was 2.0%or 3.0%,and the best time for seed sterilization with 3.0%NaClO was 15 min,causing the 97.4%of germination rate and 85.9%of healthy shoot rate with least rate of colonies.Moreover,this method of seed sterilization is applicable to other 9 plants,especially for B.napus,B.pekinensis and Arabidopsis thaliana.The root regenerated best in the rooting medium containing 0.1 mg·L^−1 NAA,in which 92.5%of root regenerated from the cutting point on the 7th day with the most lateral roots.【Conclusion】This study optimized the method of seed sterilization and the formula of rooting medium,which laid a foundation for efficient plant tissue culture of B.napus.
作者
傅淑
刘昭霞
陈金芝
孙庚晓
孙翠英
陈进
杨广
FU Shu;LIU Zhao-xia;CHEN Jin-zhi;SUN Geng-xiao;SUN Cui-ying;CHEN Jin;YANG Guang(State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/Institute of Applied Ecology,Fuzhou,Fujian 350002,China;Joint International Research Laboratory of Ecological Pest Control,Ministry of Education,Fuzhou,Fujian 350002,China;Key Laboratory of Green Control of Insect Pests(Fujian Province University),Fuzhou,Fujian 350002,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2019年第12期1371-1378,共8页
Fujian Journal of Agricultural Sciences
基金
福建省科技重大专项（2018NZ0002）
福建农林大学科技创新专项基金（CXZX2018091）
