摘要
为防控水仙黄条病毒(narcissus yellow stripe virus,NYSV)和水仙退化病毒(narcissus degen‐eration virus,NDV),于2017年4月在江苏省2个市采集24个水仙病样,通过逆转录聚合酶链式反应(reverse transcription-PCR,RT-PCR)检测,从6个水仙样品中获得与预期大小一致的核苷酸片段,通过克隆CP基因和序列分析,结合GenBank中已报道的相关序列对所获得的4条NYSV序列和2条NDV序列进行种群结构及遗传多样性分析。结果显示,仅在NYSV CP基因中发现4个重组位点;NYSV CP多样性程度很高,相似值高于75%,而NDV CP相似值高达96%;在系统发育树中,NYSV分离物被分为4组,NDV分离物被分为2组;日本NYSV分离物核苷酸多样性较高,为0.136,而NDV中国种群核苷酸多样性较高,为0.012;NYSV CP基因中II组与III组的遗传距离最高,为0.332,NDV中国分离物与日本分离物间的遗传距离差异明显,为0.104;NYSV中国种群与日本种群的遗传差异为0.166,存在一定的基因交流;NYSV与NDV的CP基因均处于负选择压力作用下。
In order to prevent and control narcissus yellow stripe virus(NYSV) and narcissus degeneration virus(NDV),24 narcissus samples were collected in two cities in Jiangsu Province in April 2017.The nucleic acid fragments with the expected size were obtained from six narcissus samples by reverse transcription polymerase chain reaction(RT-PCR) detection.The CP gene was cloned and sequenced,and the population structure and genetic diversity of four NYSV sequences and two NDV sequences combined with the related sequences reported in GenBank were analyzed.The results of recombination analysis showed that four recombination sites were found in NYSV CP gene.The diversity of NYSV CP was very high,and its similar value was higher than 75%;the similar value of NDV CP was as high as 96%.NYSV was divided into four groups,and NDV was divided into two groups in the phylogenetic trees.Nucleotide diversity of NYSV isolates in Japan was as high as 0.136,while that of NDV Chinese population was as high as 0.012;the genetic distance between group II and group III of NYSV CP gene was the highest(0.332),and the genetic distance between NDV Chinese and Japanese isolates was obviously different(0.104);the genetic difference between NYSV Chinese population and Japanese population was 0.166,with a certain degree of gene flow.The CP genes of NYSV and NDV were under negative selection pressure.
作者
甘海锋
陈雯
陈夕军
张坤
贺振
GAN Haifeng;CHEN Wen;CHEN Xijun;ZHANG Kun;HE Zhen(School of Horticulture and Plant Protection,Yangzhou University,Yangzhou 225009,Jiangsu Province,China)
出处
《植物保护学报》
CAS
CSCD
北大核心
2020年第1期187-196,共10页
Journal of Plant Protection
基金
中央本级重大增减支项目(2060302-1904-11)
国家自然科学基金(31601604)
江苏省重点研发计划(现代农业)项目(BE2018359)
关键词
水仙黄条病毒
水仙退化病毒
种群结构
遗传多样性
narcissus yellow stripe virus
narcissus degeneration virus
population structure
genetic diversity
