摘要
目的研究环境温度对按蚊繁殖能力的影响及其分子机制。方法在16、20、24、28、32℃5个温度梯度下进行斯氏按蚊的饲养,比较分析不同温度下斯氏按蚊的产卵能力和生存时间。并在饲血前后不同时间点提取斯氏按蚊总RNA,利用RT-qPCR的方法检测按蚊卵黄蛋白原(Vg)分子转录水平。结果在16℃的环境温度下斯氏按蚊停止繁殖,20℃的产卵率明显低于24、28和32℃(P<0.05),其余各组间的产卵率比较则无统计学差异。在20~28℃温度范围内,随着环境温度升高斯氏按蚊的产卵时间逐渐缩短(P<0.001)、产卵数量逐渐增高(P<0.05,P<0.01)、生存时间逐渐延长(P<0.05,P<0.01);当环境温度升至32℃时,与28℃比较,斯氏按蚊的产卵时间进一步缩短(P>0.05),但产卵数量明显减少(P<0.01),且生存时间明显缩短(P<0.01)。Vg转录水平在16℃下吸血前后始终处于基础低表达水平,其他各组在吸血后24 h显著升高,各温度间转录水平有统计学差异(P<0.01),且与产卵数量差异趋势一致。结论 28℃是斯氏按蚊繁殖和生存的最佳环境温度,其分子机制可能是通过温度影响卵黄蛋白原表达水平而实现的。
Objective To determine the effect of ambient temperature on fecundity of Anopheles stephensi and investigate its molecular mechanisms. Methods After Anopheles stephensi were divided into 5 groups, they were maintained in a mosquito cage at environment temperature of 16, 20, 24, 28 and 32 ℃, respectively. The oviposition capacity and survival time of Anopheles stephensi at different temperatures were analyzed and compared. The total RNA of Anopheles stephensi was extracted at different time points before and after blood feeding, and the transcription level of vitellogenin(Vg) was detected by RT-qPCR. Results Under the temperature of 16 ℃, Anopheles stephensi stopped reproducing. The oviposition rate was significantly lower in the 20 ℃ group than the 24, 28 and 32 ℃ groups(P<0.05). While, no such significant difference was found among the other groups. Under the range of 20~28 ℃, with the ambient temperature rising, the gravidity time was gradually shortened(P<0.001), the counts of laid eggs was gradually increased(P<0.05 or 0.01), and the survival time was gradually extended(P<0.05 or 0.01). However, when the ambient temperature rose to 32 ℃, the gravidity time was further shortened, but less eggs were laid(P<0.01) and the survival time was reduced(P<0.01), when compared with the values in the 28 ℃ group. The transcriptional level of Vg was always at low basic level before and after blood feeding under 16 ℃. Among the other temperature groups, the level was increased significantly at 24 h post blood feeding than before(P<0.01). The pattern of Vg levels among different groups was consistent with those of egg counts under the corresponding temperature. Conclusion 28 ℃ is the optimal temperature for the fecundity and survival of Anopheles stephensi. The molecular mechanism may be due to temperature affecting the expression of Vg.
作者
秦杰
于莎莎
王盼
刘婷婷
王静
王英
QIN Jie;YU Shasha;WANG Pan;LIU Tingting;WANG Jing;WANG Ying(Department of Tropical Medicine,Faculty of Military Preventive Medicine,Army Medical University(Third Military Medical University),Chongqing,400038,China)
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2020年第3期241-245,共5页
Journal of Third Military Medical University
基金
国家自然科学基金(81702035,81971971)
重庆市自然科学基金(CSTC2018jcyjAX0182)~~
关键词
温度
斯氏按蚊
繁殖能力
卵黄蛋白原
temperature
Anopheles stephensi
fecundity
vitellogenin
