摘要
MYB蛋白是一类广泛存在于真核生物中的转录因子,在真菌的生长发育中发挥调控作用。本研究对冬虫夏草菌中的潜在MYB转录因子家族基因进行了全基因组鉴定和生物信息学分析,并研究了MYB家族成员在冬虫夏草生长发育不同阶段和不同部位的表达模式。结果表明,冬虫夏草MYB转录因子家族包含3个1R-MYB和3个2R-MYB;6个MYB转录因子蛋白均包含近50个保守氨基酸序列,形成螺旋-转角-螺旋的结构。通过分析MYB转录因子基因在不同发育阶段(MYB-1–6)和子实体不同部位(MYB-1、3、6)的相对表达量,发现MYB-1在冬虫夏草整个发育阶段表达较为稳定,MYB-3在子实体成熟阶段(MF)表达量最高,远高于其他阶段,且在MF的顶部可育部分MF-3高表达,表明其可能参与冬虫夏草子实体的有性发育;MYB-6在子座发育初期(YF)的中部YF-2表达量最高,为菌丝阶段(HY)的5倍,表明MYB-6可能参与子座柄部的伸长。
The MYB family of transcription factors is widely distributed in all eukaryotic organisms,which have been shown to play an important role in the growth and development of fungi.In this study,genome-wide identification and bioinformatics analysis of MYB transcription factor family were performed,and the expression patterns of MYB family members at different development stages and different parts of fruiting body of the caterpillar fungus Ophiocordyceps sinensis were studied.The results showed that there were three 1R-MYBs and three 2R-MYBs in the genome of O.sinensis.They also contained nearly 50 conserved amino acid sequences giving rise to a helix-turn-helix(HTH)secondary structure.The relative expression levels of MYB transcription factors at different development stages(MYB-1–6)and different parts(MYB-1,3,6)of the fruiting body were studied.MYB-1 expressed constitutively during the whole growth and development stages;MYB-3 may regulate the maturation of perithecia and ascospores;MYB-6 may play role in the elongation of fruiting body.
作者
李肖
李文佳
王芬
唐玲
钱正明
董彩虹
LI Xiao;LI Wen-Jia;WANG Fen;TANG Ling;QIAN Zheng-Ming;DONG Cai-Hong(State Key Laboratory of Mycology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;University of Chinese Academy of Sciences,Beijing 100049,China;Key Laboratory of State Administration of Traditional Chinese Medicine,Sunshine Lake Pharma Co.,LTD,Dongguan,Guangdong 523850,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2019年第12期2174-2182,共9页
Mycosystema
基金
北京市科技计划课题(Z181100002418011)~~
关键词
冬虫夏草
MYB转录因子
生长发育
表达分析
Chinese cordyceps
MYB transcription factor
growth and development
expression analysis
