摘要
采用高效液相色谱法建立冬虫夏草野生及人工繁育品的特征图谱,并同时测定4种主要核苷的含量。以尿苷、肌苷、鸟苷和腺苷等4种成分为对照品,采用Waters XSelect HSS T3 C_(18)(4.6 mm×250 mm,5μm);水(A)-乙腈(B)为流动相,梯度洗脱(0~5 min,0%B;5~15 min,0~10%B,15~30 min,10%~20%B;30~33 min,20%~50%B;33~35 min,50%~0%B;35~40 min,0%B),流速0.6 mL·min^(-1),检测波长为260 nm,柱温30℃,进样量5μL,建立冬虫夏草野生品和人工繁育品的特征图谱,并同时测定4种主要核苷的含量。冬虫夏草人工繁育品和野生品之间特征图谱和4种主要核苷含量均无显著性差异,该研究能够为冬虫夏草人工繁育品的进一步开发利用提供科学依据。
This study is to establish the HPLC specific chromatogram and determine four main nucleosides of wild and cultivated Cordyceps sinensis. Uridine, inosine, guanosine and adenosine were selected as reference substance. HPLC analysis was performed on a Waters XSelect HSS T3 C18 （4. 6 mm×250 mm, 5 μm） , with a mobile phase consisting of water（A）-acetonitrile （B） at a flow rate of 0. 6 mL. rain-l （0-5 rain,0% B;5-15 min,0%-10% B, 15-30 min,10%-20% B, 30-33 min, 20%-50% B, 33-35 rain, 50%- 0% B, 35-40 min, 0% B）. The detection wavelength was 260 nm and the column temperature was controlled at 30 ~C, and the injec- tion volume was 5 μL. HPLC specific ehromatogram of wild and cultivated C. sinen,~is was established and four main nucleosides were simultaneously determined by the above method. Specific chromatograms and contents of four main nucleosides showed no significant differences between cultivated and wild C. sinensis. These results can provide scientific evidences for further development and utiliza- tion of cultivated C. sinensis.
作者
昝珂
黄莉莉
过立农
刘杰
郑健
马双成
钱正明
李文佳
ZAN Ke HUANG Li-li GUO Li-nong LIU Jie ZHENG Jianl MA Shuang-cheng QIAN Zheng-ming LI Wen-jia(National Institutes for Food and Drug Control, Beijing 100050, China Guangxi Institute for Food and Drug Control, Nanning 530021, China Sunshine Lake Pharma Co. , Ltd. , Dongguan 523808, China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2017年第20期3957-3962,共6页
China Journal of Chinese Materia Medica
基金
中医药行业科研专项(201507002)
国家"重大新药创制"科技重大专项(2014ZX09304307-002)
