摘要
目的探讨血浆微小RNA-200b(miRNA-200b)与微小RNA-21(miRNA-21)在上皮性卵巢癌(EOC)中的表达及临床意义。方法选取80例EOC患者(EOC组)、46例上皮性卵巢良性肿瘤患者(良性组)及48例健康女性(对照组)作为研究对象,检测3组研究对象血浆miRNA-200b、miRNA-21水平;应用受试者工作特征(ROC)曲线评价血浆miRNA-200b、miRNA-21水平对EOC的诊断效能及对EOC患者预后的预测价值;分析miRNA-200b、miRNA-21水平与EOC临床特征的关系。结果EOC组血浆miRNA-200b、miRNA-21相对表达量均高于良性组和对照组,差异均有统计学意义(P﹤0.05)。血浆miRNA-200b、miRNA-21诊断EOC的最佳截断值分别为2.110、1.520,二者联合诊断的曲线下面积(AUC)分别大于miRNA-200b及miRNA-21单项诊断(P﹤0.05)。不同临床分期、糖类抗原125(CA125)水平及有无淋巴结转移的EOC患者血浆miRNA-200b、miRNA-21相对表达量比较,差异均有统计学意义(P﹤0.01)。死亡组EOC患者血浆miRNA-200b、miRNA-21水平均明显高于生存组,差异均有统计学意义(P﹤0.01)。血浆miRNA-200b、miRNA-21预测EOC患者预后不良的最佳截断值分别为3.680、2.410,二者联合检测的AUC分别大于miRNA-200b及miRNA-21单独检测(P﹤0.05)。结论血浆miRNA-200b和miRNA-21可作为EOC早期诊断和预后预测的分子标志物,二者联合应用能提高诊断效能以及预后预测能力。
Objective To explore the expression of plasma microRNA-200b(miRNA-200b)and microRNA-21(miRNA-21)in epithelial ovarian cancer(EOC)and their clinical significance.Method Altogether,80 cases of EOC(EOC group),46 cases with benign epithelial ovarian tumors(benign lesions group)and 48 healthy women(normal control group)were included in the analysis,the expression levels of plasma miRNA-200b and miRNA-21 in the three groups were detected;receiver operating characteristic(ROC)curves were employed to evaluate the diagnostic efficiency of plasma miRNA-200b and miRNA-21 for the prognosis of patients with EOC;besides,the relationship between expression levels of miRNA-200b,miRNA-21 and clinical pathological features of EOC was analyzed.Result Compared with benign lesions group and control group,the relative expression of plasma miRNA-200b and miRNA-21 were significantly increased in EOC group(P<0.05).Plasma miRNA-200b and miRNA-21 demonstrated the optimal cut-off values of 2.110 and 1.520 for diagnosing EOC,respectively,additionally,the area under the curve(AUC)of miRNA-200b combined with miRNA-21 was greater than that of miRNA-200b or miRNA-21 alone(P<0.05),respectively.Clinical staging,carbohydrate antigen 125(CA125)level and presence of lymph node metastasis were significantly associated with the relative expression of plasma miRNA-200b and miRNA-21 among EOC patients,indicating statistically significant difference(P<0.01).Those who died of EOC were with obviously higher plasma miRNA-200b and miRNA-21 compared to those who survived,and the difference was of statistical difference(P<0.01).The optimal cut-off values of plasma miRNA-200b and miRNA-21 for predicting poor prognosis of EOC patients were 3.680 and 2.410,respectively,AUC of miRNA-200b combined with miRNA-21 was also greater than that of miRNA-200b and miRNA-21 alone(P<0.05).Conclusion Plasma miRNA-200b and miRNA-21 can be applied as molecular markers for early diagnosis of EOC and prognosis prediction.The combination of the two can improve the diagnostic efficiency and also the prediction ability for prognosis.
作者
李恩萍
史天云
王博
张欣萍
LI Enping;SHI Tianyun;WANG Bo;ZHANG Xinping(Department of Gynecology,Nanyang First People’s Hospital,Nanyang 473010,He’nan,China)
出处
《癌症进展》
2020年第3期258-261,共4页
Oncology Progress
