摘要
目的分析南京地区儿童肺炎支原体(MP)感染现状,并对检测方法进行比较,为临床早期诊断及合理治疗提供依据。方法回顾性分析2018年1-12月于南京医科大学附属儿童医院就诊的26230例急性呼吸道感染患儿的MP抗体检测结果;对凝集法、化学发光法及酶联免疫法检测的MP抗体结果进行比较。结果入选患儿MP抗体检测的总阳性率为46.32%;凝集组、酶免组、发光组检测的MP抗体阳性率分别为53.63%、45.10%、42.06%,差异有统计学意义(P<0.05)。≥6岁患儿MP抗体阳性率最高(55.21%),0~<2岁患儿MP抗体阳性率最低(37.47%);女性患儿的MP抗体阳性率(52.52%)高于男性患儿(41.50%)。凝集法与化学发光法检测MP抗体的阳性符合率为90.27%,阴性符合率为73.21%,总符合率为87.26%。结论MP为南京地区儿童急性呼吸道感染的主要病原体之一,其感染率存在性别、年龄及季节差异。在通过血清抗体判断现症感染时,需同时检测IgM、IgG抗体以增加检测结果的准确性。
Objective To analyze the current situation of Mycoplasma pneumoniae(MP)infection in children in Nanjing area,and compare the detection methods to provide evidence for early clinical diagnosis and reasonable treatment.Methods The antibody test results of MP in 26230 children with acute respiratory infection treated at Children′s Hospital Affiliated to Nanjing Medical University from January to December 2018 were analyzed retrospectively,and the results of agglutination,chemiluminescence and ELISA were compared.Results The total positive rate of MP antibody in the children was 46.32%;the positive rates of the agglutination group,ELISA group,and chemiluminescence group were 53.63%,45.10%and 42.06%respectively,the positive rates of the 3 groups were statistically significant(P<0.05).The positive rate of MP antibody was highest in children≥6 years(55.21%),and the lowest positive rate of MP antibodies in 0 to 2 years(37.47%);The positive rate of MP antibody in female children(52.52%)was higher than that in male children(41.50%).The positive coincidence rate of MP antibody detected by agglutination and chemiluminescence was 90.27%,the negative coincidence rate was 73.21%,and the total coincidence rate was 87.26%.Conclusion MP is one of the important pathogens of children with acute respiratory infection in Nanjing area,and its infection rate varies with gender,age and season.When judging the current infection through serum antibodies,it is necessary to detect IgM and IgG antibodies at the same time to increase the accuracy of the results.
作者
高磊
高岭
GAO Lei;GAO Ling(Department of Clinical Laboratory,Nanjing Red Cross Hospital,Nanjing,Jiangsu 210001,China;Department of Clinical Laboratory,Children′s Hospital Affiliated to Nanjing Medical University,Nanjing,Jiangsu 210008,China)
出处
《检验医学与临床》
CAS
2020年第5期633-635,639,共4页
Laboratory Medicine and Clinic
关键词
肺炎支原体
儿童
流行病学
抗体分型
Mycoplasma pneumoniae
children
epidemiology
antibody typing
