摘要
细胞外三磷酸腺苷(extracellular adenosine-5′-triphosphate)是植物细胞的重要信号分子。以烟草悬浮细胞BY-2(Nicotiana tabacum L.cv.Bright Yellow-2)为材料,探讨了胞外三磷酸腺苷对铅胁迫下细胞损伤、H 2O 2(过氧化氢)含量及H 2O 2清除酶活性的影响。结果显示,随着Pb(NO 3)2浓度的不断提高(30~400μmol·L-1),细胞外三磷酸腺苷含量呈现出逐渐下降的趋势,但胞内三磷酸腺苷含量及细胞的受损伤程度逐渐增大;同时,H 2O 2含量和过氧化氢酶的活性均有所上升,并在200μmol·L-1 Pb(NO 3)2处理下达到最大值,而过氧化物酶的活性则不断降低。较之Pb(NO 3)2胁迫下的细胞,对Pb(NO 3)2胁迫的细胞加入外源三磷酸腺苷使得细胞受损伤程度显著降低,H 2O 2含量减少,过氧化氢酶活性减弱,而过氧化物酶活性增强。实验结果表明,Pb(NO 3)2胁迫诱导的植物细胞损伤和H 2O 2及其清除酶水平的变化能受到细胞外三磷酸腺苷水平的调节。
The extracellular adenosine-5′-triphosphate is an important signaling molecule for plant cells.By using the tobacco(Nicotiana tabacum L.cv.Bright Yellow-2)cell suspension cultures,we studied the effects of extracellular adenosine-5′-triphosphate on the cellular damage,H 2O content,and the activities of H 2O detoxifying[JP3]enzymes underlead stress.With the increase of concentrations of Pb(NO 3)2(from 30-400μmol·L-1),[JP]the levels of the extracellular adenosine-5′-triphosphate were decreased,whilethe levels of the intracellular adenosine-5′-triphosphate and cellular damage were increased.The content of H 2O 2 and the activity of the CAT(catalaseenzyme)were increased and reached their maximum at 200μmol·L-1 Pb(NO 3)2,while the activityof the POD(peroxide enzyme)was decreased,with the increase of concentrations of Pb(NO 3)2.Compared with the cells under lead(200μmol·L-1)stress,the lead-stressed cells treated with exogenous adenosine-5′-triphosphate(30μmol·L-1)had lower levels of cellular damage,H 2O 2 content,andthe activity of CAT,but had higher level of the activity of POD.Therefore,the cellular damage,[JP3]H 2O content,and the activities of H 2O detoxifying enzymesunderlead stress could beregulated by extracellular adenosine-5′-triphosphate.
作者
曹佳鑫
达梦婷
庞海龙
贾凌云
冯汉青
CAO Jia-Xin;DA Meng-Ting;PANG Hai-Long;JIA Ling-Yun;FENG Han-Qing(College of Life Science,Northwest Normal University,Lanzhou 730070)
出处
《植物研究》
CAS
CSCD
北大核心
2020年第1期85-92,共8页
Bulletin of Botanical Research
基金
国家自然科学基金(31870246,31560070)
甘肃省高等学校科研项目(2015A-007)
甘肃省重点研发计划项目(18YF1NA051)
甘肃省高校基本科研业务费
西北师范大学青年创新团队项目~~
