摘要
为分析炭疽菌侵染对草莓‘晶瑶’抗性生理指标的影响,研究检测了‘晶瑶’接种胶孢炭疽菌后,不同时间点的12个生理指标的变化情况。结果表明:在96 h检测范围内,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)活性以及可溶性总蛋白、维生素C、丙二醛(MDA)、木质素、总酚、绿原酸、阿魏酸含量总体表现为先升高后降低的变化趋势;MDA含量达到最高水平的时间早于PAL、总蛋白、总酚、阿魏酸,晚于SOD、POD、CAT、维生素C、木质素、绿原酸;MDA含量的提高倍数小于CAT、PAL、总蛋白,但大于其他8个抗性生理指标;高含量MDA的持续时间长于其他抗性指标。这些结果表明,炭疽菌侵染后,相对于丙二醛含量,其他抗性指标的提高幅度较小、持续时间较短,是与‘晶瑶’易感炭疽病相关的生理指标。该结果为深入研究‘晶瑶’的炭疽病发病机制以及进行诱抗等措施提高其抗性的研究提供了参考。
In order to research the influence of Anthracnose infection on the physiological factors related toresistance in a strawberry cultivar‘Jingyao＇, the changes of 12 physiological factors involved in diseaseresistance were detected at different times after inoculated by Colletotrichum gloeosporioides. The resultsshowed that the activity of superoxide dismutase（SOD）, peroxidase（POD）, catalase（CAT）, phenylalaninammonialyase（PAL）, polyphenol oxidase（PPO） and the content of malondialdehyde（MDA）, total solubleproteins, vitamin C, total phenols, lignin, chlorogenic acid（CHA）, ferulic acid（FA） were showed increased firstand then decreased in the 96 hours range. The time of the highest content of MDA was earlier than that of PAL,total soluble proteins, total phenols, FA, and later than that of SOD, POD, CAT, vitamin C, lignin, CHA. Theincreased folds of MDA content were less than that of CAT, PAL, total soluble proteins and more than that ofother 8 physiological factors. The continuance time of high MDA content was longer than other physiologicalfactors. These results suggested that the increasing extent and continuance time of other physiological factorsinvolved in disease resistance were less than that of MDA, which might be correlation to the anthracnose-susceptible character of‘Jiangyao＇. Our results laid a foundation for further research the pathogenesis of C.gloeosporioides in‘Jiangyao＇and provided a reference for research on induce resistance of‘Jingyao＇to anthracnose.
出处
《中国农学通报》
CSCD
2014年第13期161-167,共7页
Chinese Agricultural Science Bulletin
基金
国家农业科技成果转化资金项目"抗病高产草莓新品种‘晶玉’中试与示范"(2012GB2D100298)
农业部公益性行业科研专项项目"南方地区草莓品种的选育及优质高效栽培关键技术研究"(201003064)
湖北省农业科技创新中心资助项目"草莓
魔芋高效安全生产关键技术研究与应用"(2007-620-006-003)
湖北省农业科学院青年科学基金"草莓新品种‘晶玉’的抗炭疽病机理研究"(2012NKYJJ05)
关键词
草莓
炭疽病
丙二醛
防御酶
抗性物质
strawberry anthracnose malondialdehyde defense enzyme resistance substance
