摘要
以柠檬醛、香叶醇为考察指标,采用高效液相色谱法对广西、云南两地香茅草进行含量测定,并通过DPPH·法测定不同产地香茅草甲醇提取物清除自由基的能力,并计算不同产地香茅草甲醇提取物清除50%自由基时样品浓度(IC50值)。实验结果表明所用方法简便、快捷、重复性好、准确度高,可用于香茅草药材质量控制方法,云、桂两地香茅草醇提物均有清除自由基能力,具有较大的开发利用前景。
In this study, we determined geraniol and citral concentrations as well as their free radical scavenging capacity in Cymbopogon citratus from Guangxi and Yunnan provinces by high-performance liquid chromatography(HPLC) method. The scavenging activity was obtained by the methanol extracts of Cymbopogon citratus against DPPH free radical and calculated by removing 50% of free radical concentration(IC50) by Cymbopogon citratus from different sources. The developed method from the present work is simple, quick, reproducible and accurate, which should be helpful for the quality control of the Cymbopogon citratus. In addition, we established that the methanol extracts of Cymbopogon citratus from different sources possess considerable scavenging effect on DPPH, demonstrating a great potential of the development value.
作者
梁芳
叶绘晟
龚志强
杨力龙
蒙田秀
LIANG Fang;YE Hui-sheng;GONG Zhi-qiang;YANG Li-long;MENG Tian-xiu(Faculty of Chinese Medicine Science,Guangxi University of Chinese Medicine,Nanning 530222,China;The First Affiliated Hospital,Guangxi University of Chinese Medicine,Nanning 530023,China)
出处
《晓庄学院自然科学学报》
CAS
北大核心
2020年第1期63-67,共5页
Journal of Natural Science of Hunan Normal University
基金
广西壮族自治区教育厅科研课题资助项目(2013LX064,YB2014616)
