摘要
为提高虫草素的产量,本实验对蛹虫草固态发酵产虫草素进行优化。通过一系列单因素实验,确定大米为发酵基质,葡萄糖和黄豆粉分别为最适碳源和氮源,得到最佳培养基组成和最佳培养条件:大米30 g(粒径0.90~1.25 mm),料液比(m/v)1∶1.5,葡萄糖3%(按基质算,下同),黄豆粉2%,麦麸1%,接种量30%,种龄2 d,发酵时间12 d。优化后虫草素产量达到4.69%,约为优化之初(0.74%)6.34倍。
To improve the production of cordycepin,the experiments were conducted to optimize the production of cordycepin by solid-state fermentation of Cordyceps militaris.Through a series of single-factor experiments,rice was the fermentation substrate,glucose and soybean flour were the optimum carbon source and nitrogen source respectively,the optimum medium compositions and culture conditions were:rice 30 g(particle size 0.90~1.25 mm),solid-liquid ratio(m/v)1∶1.5,glucose 3%(based on substrate,the same below),soybean flour 2%,wheat bran 1%,inoculation amount was 6 mL,seed age was 2 d,fermentation time was 12 d.The optimization results are that cordycepin production reached 4.69%,which is about 6.34 times compared with the initial level(0.74%).
作者
杨帆
刘金彬
管政兵
廖祥儒
蔡宇杰
YANG Fan;LIU Jinbin;GUAN Zhengbing;LIAO Xiangru;CAI Yujie(Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2019年第10期30-36,共7页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(21275066)
关键词
蛹虫草
固态发酵
虫草素
Cordyceps militaris
solid-state fermentation
cordycepin
