摘要
目的探讨白介素18(interlekin18,IL-18)和炎症小体(inflammasome)在大肠腺瘤性息肉中的表达及其临床意义。方法选取本院20例大肠息肉患者,根据其病理诊断分为非腺瘤息肉组、腺瘤性息肉组各10例,对照组为健康体检志愿者10例,收集入选者的一般资料,用酶联免疫吸附试验(enzymelinked immunosorbent assay,ELISA)检测血清中IL-18、IL-1β的含量,用免疫组化染色(immunohistochemical,IHC)观察各组患者肠息肉中IL-18、Ki-67的表达水平,蛋白印迹实验(western blot,WB)检测患者肠息肉组织中IL-1β、NLRP3、Caspase-1、GAPDH的表达量,比较分析各组患者间上述指标的差异。结果腺瘤性息肉组与非腺瘤息肉组间的性别、年龄、病程、临床症状表现无统计学差异。ELISA结果提示腺瘤性息肉患者血清中IL-18表达水平比对照组、非腺瘤息肉组明显上调(P=0.0008、0.0036),而非腺瘤息肉与对照组血清IL-18表达无差异;IL-1β在三组血清中的表达无明显差异。免疫组化染色显示腺瘤性息肉组的息肉组织中IL-18、Ki-67表达较其他两组明显增强。WB结果提示腺瘤性息肉组的IL-1β、NLRP3、Activecaspase-1的表达较非腺瘤性息肉组、对照组明显上调。结论IL-18和炎症小体在腺瘤性息肉患者的血清和肠道息肉组织中表达明显上调,说明NLRP3炎症通路、ASC-Caspase-1-IL-18/IL-1β通路在腺瘤性息肉形成过程中被激活,提示IL-18、炎症小体在肠道炎性增生性息肉转变腺瘤性息肉过程中可能发挥着重要作用。
Objective To investigate the expression and clinical significance of Interlekin18 and Inflammasome in intestinal adenomatous polyps.Methods Twenty patients with colorectal polyps in our hospital were selected and divided into non-adenomatous polyp group and adenomatous polyp group according to their pathological diagnosis,and the control group was composed of 10 healthy volunteers.General data of the selected subjects were collected.The contents of IL-18 and IL-1βin serum were detected by enzyme-linked immunosorbent assay(ELISA),and the expression levels of IL-18 and Ki-67 in intestinal polyps of the patients in each group were observed by Immunohistochemical staining(IHC).Western Blot(WB)was used to detect the expressions of IL-1β,NLRP3,Caspase-1 and GAPDH in the intestinal polyp tissues of patients,and the differences in the above indicators between the groups were compared and analyzed.Results There were no statistically significant differences between the adenomatous polyp group and the non-adenomatous polyp group in gender,age,course of disease and clinical symptoms.The expression level of IL-18 in serum of patients with adenomatous polyps was significantly higher than that of control group and non-adenomatous polyp group(P=0.0008<0.001),adenomatous polyp group and non-adenomatous polyp group(P=0.00036<0.01),while there was no difference in serum IL-18 expression between non-adenomatous polyps and control group.And there was no significant difference in serum IL-1βexpression among the three groups.Immunohistochemical staining showed that the expressions of IL-18 and Ki-67 in the polyp tissues of adenomatous polyp group were significantly enhanced compared with the other two groups.WB results indicated that Il-1β,NLRP3,and active-caspase-1 were significantly up-regulated in the adenomatous polyp group compared with the non-adenomatous polyp group and the control group.The results of WB show that the expression of IL-1β,NLRP3,and Active-caspase-1 in intestinal polyp mucosa was significantly enhanced in the adenomatous polyp group.Conclusion IL-18 in the adenomatous polyp patients serum and intestinal mucosal tissues express raised obviously,indicating that the NLRP3 inflammatory pathway and the ASCCaspase-1-IL-18/IL-1βpathway were activated during the formation of adenomatous polyps,and suggesting that IL-18 and inflammasome may play an important role in the transformation of intestinal inflammatory proliferative polyps into adenomatous polyps.
作者
贺芝兰
谢文瑞
何君连
晏杰
陈垦
冯致婷
HE Zhilan;XIE Wenrui;HE Junlian;YAN Jie;CHEN Ken;FENG Zhiting(Department of Clinical Medicine,Guangdong Pharmaceutical University,Guangzhou 510310,China;不详)
出处
《实用医学杂志》
CAS
北大核心
2019年第23期3644-3649,共6页
The Journal of Practical Medicine
基金
广东省教育厅重大科研项目（编号：2018KZDXM057）
广州市科技计划项目（编号：201804020042）
广东药科大学校级教育教学改革项目（编号：广药大教[2016]10号）
