摘要
目的:COX-2高表达与肿瘤发生的密切关系被多数学者证实,并且非甾体消炎药已被用来预防或治疗结肠多发息肉和结肠癌。GAS除了促进胃粘膜生长外,亦发现对结肠粘膜细胞的生长有刺激作用,而且近年研究表明GAS的这种促生长作用与COX-2的表达密切相关。本文旨在探索GAS、COX-2的表达与结肠腺瘤性息肉组织增殖、分化以及是否恶变之间的联系。方法:回顾性收集2003年6月～2006年6月间合肥医学院附属医院行结肠镜下息肉切除、外科手术和取病理活检的息肉标本,结合其临床资料,共获取腺瘤性息肉标本68例,另选10例非腺瘤性息肉和15例肠镜检查未见异常的肠粘膜组织作为正常对照组。所有患者均无家族性腺瘤病史和结肠癌史,均未长期服用过非甾体抗炎药(NSAIDs)、质子泵抑制剂或糖皮质激素。通过免疫组化染色,观察不同组织学类型、不同异型增殖程度以及有无局部恶变时息肉中GAS、COX-2的表达情况,以Ki-67的表达情况作为判断组织增殖程度的指标。结果:GAS、COX-2在正常肠粘膜及非腺瘤息肉中呈弱阳性表达或不表达,在绒毛腺瘤、重度异型增生及腺瘤局部恶变时高表达。其表达与组织类型和异型增生程度均有直线相关性(P<0.01)。COX-2的阳性表达与GAS表达相关(r=0.67,P<0.01)。Ki-67表达在GAS或COX-2增高时亦增高,特别在GAS和COX-2共同表达时增加明显。结论:GAS、COX-2的表达在绒毛腺瘤、重度异型增生及腺瘤局部恶变时呈高表达,与组织的增生程度和息肉恶变的危险性之间具有相关关系,GAS高表达时COX-2表达增高,同时Ki-67表达亦增高。提示GAS、COX-2在结直肠腺瘤性息肉发生、发展及恶变过程中扮演重要的角色。
Objective： To investigate the relationship between gastrin and cyelooxygenase-2（COX-2） expression and proliferation, differentiation and malignant transformation of colorectal adenomas. Methods： The expression of gastrin, COX-2 and Ki-67 was detected by immunohistoehemistry in 68 samples of coloreetal adenoma, 10 samples of non-adenoma and 15 samples of normal colonic mueosa. Those patients with intlammalory bowel disease, familial polyposis eoli or a history of taking non-steroidal anti-inflammatory drugs （including low-dose aspirin） or glucoeorticoid and proton pump inhibitors were excluded. Results： The expression of gastrin and COX-2 was higher in adenoma tissues （P〈0.01）, especially in villous adenoma, severe dysplasia and polyps with malignant transformation. Correlation analysis showed that gastrin and COX-2 expression levels were significantly related to pathological type and dysplasia grade of polyps （P〈0.01）. Furthemore, a positive correlation between COX-2 and gastrin expression （r=0.67. P〈0.01） was found. The expression of Ki- 67 was significantly higher in patients with expression of both gastrin and COX-2 than in patients without expression of gastrin or COX-2 （P〈0.01）. Conclusion： Increased expression of gastrin and COX-2 is involved in the mechanisms of growth and malignant transformtion of adenoma, and it may play an important role in the progression of colorectal adenoma. Gastrin may induce COX-2 expression and co-stimulate cell proliferation during the growth and malignant transformation of adenoma.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2008年第4期206-209,共4页
Chinese Journal of Clinical Oncology
基金
贵州省优秀教育科技人才省长基金资助(编号:2005-82)
