摘要
目的通过对感染泡球蚴的小鼠肝脏进行自噬相关Beclin1基因mRNA及其蛋白的检测,了解自噬在泡球蚴感染过程中的变化。方法取雌性BALB/c小鼠32只,随机分为实验组与对照组。实验组建立泡球蚴感染小鼠模型,于感染30 d和90 d各处死8只,通过HE染色观察小鼠肝脏的病理学变化,利用qRT-PCR、免疫组化等技术分别检测泡球蚴感染小鼠肝脏中Beclin1基因mRNA及蛋白的表达。结果实验组小鼠肝脏Beclin1基因mRNA及蛋白表达均增高,其中感染30 d和90 d Beclin1基因mRNA及蛋白相对表达量分别为2.34±0.59、14.19±3.49和1.69±0.59、9.23±1.37,差异均有统计学意义(P<0.05);与对照组同期比较差异均有统计学意义(P<0.05)。且肝组织有典型囊泡病灶。结论泡球蚴感染小鼠肝脏中Beclin1基因高表达,且以感染30d时增高更显著,提示在泡球蚴感染早期和中期,自噬随着感染呈现先增强再减弱的趋势。
Objective To explore changes in autophagy during the process of infection by detecting autophagy-related gene Beclin1 mRNA and its protein in the livers of mice infected with Echinococcus multilocularis.Methods Thirty-two female BALB/c mice were randomly divided into an experimental group and a control group.In the experimental group,a mouse model of E.multilocularis infection was created;8 mice were sacrificed after 30 and 90 d.Pathological changes in the livers of mice were observed using HE staining.qRT-PCR and immunohistochemistry techniques were used to detect the expression of Beclin1 gene mRNA and protein in the livers of mice infected with E.multilocularis.Results The expression of Beclin1 mRNA and protein increased in the livers of mice in the experimental group mice,and the relative expression of Beclin1 mRNA and protein was 2.34±0.59 and 14.19±3.4930 d after infection and 1.69±0.59 and 9.23±1.3790 d after infection.Levels of expression differed significantly(P<0.05)compared to levels in the control group(P<0.05).Liver tissue had typical vesicular lesions.Conclusion The Beclin1 gene is highly expressed in the liver of mice infected with E.multilocularis,and the increase was more significant 30 days after infection.This suggests that autophagy tends to first increase and then decrease during the early and middle stages of E.multilocularis infection.
作者
木合塔尔·色特瓦勒迪
蔡璇琳
赵慧
叶门勒汉·巴合苏提汉
李斌
克迪尔叶·开塞尔
孟星
古力米热木·买买提江
吕洁
马秀敏
Muhetaer·Setewaledi;CAI Xuan-lin;ZHAO Hui;Yemenlehan·Bahesutihan;LI Bin;Kedierye·Kaisaier;MENG Xing;Gulimiremu·Maimaitijiang;LV Jie;MA Xiu-min(First Hospital Affiliated with Xinjiang Medical University,Urumqi,830011;State Key Laboratory of Pathogenesis,Prevention and Treatment of Highly Prevalent Diseases in Central Asia,First Hospital Affiliated with Xinjiang Medical University;College of Basic Medicine,Xinjiang Medical University)
出处
《中国病原生物学杂志》
CSCD
北大核心
2019年第11期1293-1296,共4页
Journal of Pathogen Biology
基金
大学生创新训练计划项目(No.CX2018003)
国家自然科学基金项目(No.81760372)
新疆自治区自然科学基金项目(No.2016D01C325)
