摘要
目的探究红树莓提取物对ApoE-/-小鼠动脉粥样硬化及NF-κB/VCAM-1通路的影响。方法本研究中,选取66只ApoE-/-小鼠,并随机分为A,B,C,D,E,F组,每组11只,其中A组为对照组,每日给予普通饲料喂养3周;B为模型组,C到E组分别为红树莓低浓度、中浓度及高浓度组,F组为阿托伐他汀钙片组,在HFD(脂肪21%,蛋白质20%,胆固醇0.15%)条件下饲养3周。3周后,对照组以及模型组给予等量蒸馏水喂养,C-E组通过灌胃方式给予不同浓度的红树莓提取物,F组给予0.35mg/kg•d的等量阿托伐他汀钙片。12周后抽取小鼠血液样本,检测血清总胆固醇(TC)、三酰甘油(TAG)、NO、诱导型一氧化氮合成酶(iNOS)、还原性谷胱甘肽抗氧化酶(GSH)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)、过氧化氢酶(CAT)的浓度及活性,通过western-blotting检测主动脉组织中NF-κB、TNF-α及血管细胞黏附因子-1(VCAM-1)的蛋白表达水平;对主动脉血管组织进行HE染色以及免疫组化检测,观察主动脉粥样硬化斑块形成状况。结果通过对实验组以及对照组主动脉粥样硬化红油O染色以及免疫组化结果观察发现,C-E组的小鼠主动脉粥样硬化病变程度显著下降,可见血管壁厚度以及斑块显著减少,且随着红树莓提取物浓度提高血管壁厚度以及斑块同样减少,但无显著差异;与模型组B相比,C-E组的TC、TAG水平有所下降,NO含量以及iNOS酶活性显著增加,并且随着红树莓提取物浓度升高小鼠体内NO含量以及iNOS酶活性呈现升高趋势且与阿托伐他汀钙片组之间并无显著差异,;C-E组小鼠体内GSH,SOD以及GPx活性均显著高于模型组。与模型组B相比,C-E组的NF-κB、TNF-α及VCAM-1蛋白表达明显降低。结论红树莓提取物可以通过NO系统提高小鼠体内抗氧化物质含量改善动脉粥样硬化。
Objective To investigate the effects extract of red raspberry and NF-κB/VCAM-1 pathway on atherosclerosis in ApoE knockout mice.Methods Total of 66 ApoE knockout mice was selected and divided them into A,B,C,D,E,F groups and randomly with 11 mice in each group.The group A was the control group,which was fed with normal diet.The group B was the model group with high fat diet.The group C to E were the low,medium and high concentration groups of red raspberry receiving a high fat diet.And the group F was the atorvastatin calcium group which was also fet in a high fat diet.After 3 weeks,the control group A and the model group B were taken the same amount of distilled water.The red raspberry groups C to E were given different concentrations of freshly prepared red raspberry extract by gavage.The mice in group F were taken the equal amount of atorvastatin calcium tablets at 0.35 mg/kg/d.After 12 weeks,the blood samples were taken from each group to test the serum total cholesterol(TC),triglyceride(TAG),NO,inducible nitric oxide synthase(iNOS),reduced glutathione antioxidant enzyme(GSH),superoxide dismutase(SOD)and glutathione peroxidase(GPx).The expression ofNF-κB,TNF-αand vascular cell adhesion factor-1(VCAM-1)in aortic tissues were detected by western-blotting(WB)test.The formation of atherosclerotic plaques was observed by HE staining and immunohistochemistry.Results The results of aortic atherosclerosis HE staining and immunohistochemistry shown that the degree of aortic atherosclerotic lesions in the experimental group was significantly decreased,and the thickness of the vessel wall and the plaque were reduced.With the increase of the concentration of raspberry extract,the thickness of vessel wall and plaque also decreased.Compared with model group B,the TC and TAG levels of experimental group C-E were decreased,the NO content and iNOS activity were increased significantly.The activities of GSH,SOD and GPx in mice of experimental group were significantly higher than those of model group.Compared with model group B,the expressions of NF-kappa B,TNF-alpha and VCAM-1 in C-E group were significantly lower.Conclusion Red raspberry extract can improve the body of mice through NO system.Antioxidant levels improve atherosclerosis.
作者
钱银芬
罗红阳
钱伟
付茂蓉
曹磊
罗瑞
杨永曜
Qian Yinfen;Luo Hongyang;Qian Wei;Fu Maorong;Cao Lei;Luo Rui;Yang Yongyao(Wudang District People’s Hospital of Guiyang City,Guiyang 550018,Guizhou,China;Guizhou Provincial People’s Hospital,Guiyang 550002,Guizhou,China)
出处
《贵州医药》
CAS
2019年第12期1854-1858,共5页
Guizhou Medical Journal
基金
贵阳市科技局项目(筑科合同[2017]29-5号)
国家临床重点专科建设项目[No(2013)554]
贵州省科学技术厅临床研究中心资助项目[黔科合平台人才2017(5405)]
