摘要
目的探讨戊酸雌二醇(E2V)对多囊卵巢综合征(PCOS)大鼠氯米芬促排卵子宫内膜容受性的改善作用。方法将大鼠分为对照组(0.9%氯化钠溶液灌胃)、PCOS模型组(50 mg/kg氯米芬灌胃)、E2V低、中和高剂量干预组[(0.2、0.5、1.0)mg/kg E2V+50 mg/kg氯米芬灌胃]。用ELISA检测血清性激素水平;观察子宫内膜形态学变化;用RT-qPCR和Western blot检测子宫内膜中雌激素受体(ER)、孕激素受体(PR)、同源框基因(Hox)A10、空通气孔同源框(Emx)2、整合素(integrin)αvβ3 mRNA和蛋白表达情况。结果与对照组相比,PCOS模型组大鼠黄体生成激素/卵泡生成激素(LH/FSH)、睾酮(T)水平、Emx2 mRNA和蛋白相对表达量均明显升高(P<0.05),雌二醇(E2)水平、腺体面积、腺腔面积、子宫内膜厚度及腺/间质面积比、ER、PR、HoxA10、integrinαvβ3 mRNA和蛋白相对表达量均明显降低(P<0.05);与PCOS模型组相比,E2V低、中和高剂量干预组上述指标变化均相反(P<0.05)。结论 E2V可改善PCOS大鼠的氯米芬促排卵子宫内膜容受性,推测与上调ER、PR、HoxA10、integrinαvβ3 mRNA和蛋白表达,下调Emx2 mRNA和蛋白表达有关。
Objective To investigate the effect of estradiol valerate(E2V) on endometrial receptivity induced by clomiphene in polycystic ovary syndrome(PCOS) rats. Methods Rats were divided into control group(administered 0.9% sodium chloride solution), and PCOS model group(administered 50 mg/kg clomiphene), E2V low, medium and high-dose intervention group [administered(0.2, 0.5, 1.0)mg/kg E2V+50 mg/kg clomiphene]. Serum sex hormone level was compared. Morphological change of endometrium was examined by ELISA. The expression of estrogen receptor(ER), progesterone receptor(PR), homeobox gene(Hox)A10, empty ventilator homeobox(Emx)2, integrin αvβ3 mRNA and protein in the endometrium were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR) and Western blot. Results Compared with the control group, luteinizing hormone/follicle-forming hormone(LH/FSH), testosterone(T), relative expressions of Emx2 mRNA and protein were significantly increased(P<0.05), while estradiol(E2) level, glandular area, glandular area,endometrial thickness and glandular/interstitial area ratio, relative expressions of ER, PR, HoxA10, integrin αvβ3 mRNA and protein were significantly decreased(P<0.05). Compared with PCOS model group, the changes of above indexes in E2V low, medium and high-dose intervention group were opposite(P<0.05). Conclusions E2V can improve endometrial receptivity of PCOS clomiphene-induced ovulation rats. E2V is potentially related to the up-regulation of ER, PR, HoxA10, integrinαvβ3 and down-regulation of Emx2.
作者
邵锐锋
何驰华
王莹
SHAO Rui-feng;HE Chi-hua;WANG Ying(Department of Reproductive Medicine,Jingzhou Central Hospital,Jingzhou 434000,China)
出处
《基础医学与临床》
CSCD
2020年第1期78-82,共5页
Basic and Clinical Medicine
基金
荆州市课题基金({2015}24号-48)
