摘要
文章通过同源克隆从番茄叶片中克隆到SlCDT1a基因,亚细胞定位结果显示SlCDT1a定位在细胞核中。此外,构建SlCDT1a过表达载体,并利用农杆菌介导法转化番茄得到SlCDT1a过量表达植株。番茄中的CUL4-DDB1-DET1作为泛素连接酶参与许多重要的生长发育过程,包括细胞分裂等。该研究利用免疫共沉淀实验发现SlCDT1a与CUL4、DDB1和DET1均有较强的相互作用,结果表明番茄SlCDT1a基因可能参与细胞有丝分裂;半定量聚合酶链式反应(polymerase chain reaction, PCR)分析结果发现,SlCDT1a过表达植株T1代中细胞周期Cyclin B基因转录水平明显高于野生型,该结果进一步表明了SlCDT1a在细胞周期中可能起到的关键作用。研究结果为深入理解SlCDT1a在番茄生长发育过程中的作用奠定了重要基础。
In this paper, tomato SlCDT1a gene was cloned by homologous cloning. Subcellular localization analysis found that SlCDT1 a was located in nucleus. We constructed SlCDT1a overexpression vector and transformed it in tomato by agrobacterium-mediated method. In tomato, CUL4-DDB1-DET1, as a ubiquitin E3 ligase complex, could be involved in many important development processes including cell division. Co-immunoprecipitation analysis showed that SlCDT1 a could interact with CUL4, DDB1 and DET1. This result suggested SlCDT1a might be involved in cell cycle. Moreover, semi-quantitative polymerase chain reaction(PCR) results showed that the transcription level of Cyclin B(CYCB) gene was upregulated in the T1 generation of SlCDT1a overexpression plants compared to wild type. It indicated that SlCDT1 a might play an important role in cell cycle. These results lay a foundation for understanding the mechanism of SlCDT1a in the growth and development of tomato plants.
作者
胡鑫
刘永胜
唐晓凤
HU Xin;LIU Yongsheng;TANG Xiaofeng(School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China)
出处
《合肥工业大学学报（自然科学版）》
CAS
北大核心
2019年第12期1704-1708,1724,共6页
Journal of Hefei University of Technology：Natural Science
基金
国家自然科学基金青年科学基金资助项目(31701922)
中央高校基本科研业务费专项资金资助项目(113-4115104003)
