摘要
目的:观察自闭症模型大鼠的痛觉时反应量-效关系特征。方法:取10只孕鼠随机分为正常孕鼠组(n=4)和模型孕鼠组(n=6),其中模型孕鼠组孕12.5 d用丙戊酸钠600 mg/kg一次性腹腔注射制备自闭症模型。在孕鼠生产后取全部子代雄性幼鼠分别为正常组22只和模型组24只,进行行为学检测鉴定模型,并进一步检测子代大鼠的机械刺激缩足反应阈值(PWMT)和不同光强度下的甩尾反射潜伏期(TFL)。结果:模型组大鼠的生长发育指标和探究行为能力均低于正常组(P<0.01);模型组大鼠的PWMT较正常组升高(P<0.01);2组大鼠的TFL均呈现随光刺激强度增强而缩短的时反应量-效关系(TDRR,P<0.01),其中模型组大鼠的TDRR曲线较正常组右移(P<0.01),并模型组的TFL较正常组延长(P<0.01)。结论:自闭症模型大鼠的机械痛和热辐射痛均较正常组大鼠耐受,并且其TFL的TDRR曲线也较正常组明显右移。
AIM:To observe the characteristics of timed dose-response relationship(TDRR)of pain in autistic model rats.METHODS:Ten pregnant SD rats were randomly divided into model pregnant group(n=6)and normal pregnant group(n=4).The model pregnant group rats were prepared by intraperitoneal injection of sodium valproate 600 mg/kg at 12.5 days after pregnancy and equivalent volume of saline was used as control injection in normal pregnant group.Then their offspring were used as model group(n=24)and normal group(n=22),respectively.During the period of 35 days after birth,a series of behavior tests were measured to identify the autistic model rats.Thereafter,the paw withdrawal mechanical threshold(PWMT)and tail flick latency(TFL)evoked by light radiant heat were measured from these rats in two groups.RESULTS:The growth and development indicators and the ability of behavioral exploration in model group rats were both less than those in normal group(P<0.01).Compared with normal group rats,the PWMT of the model group rats increased significantly(P<0.01).TFLs of rats in two groups were both shortened with increased light intensities,indicating a hyperbolic type-like TDRR.However,the TFLs in the TDRR curve of model group rats were longer than those of normal group rats(P<0.01).CONCLUSION:These preliminary results suggest that the autistic model rats increase the threshold of mechanical and heat pain,and change the TDRR curve of TFL to right either.
作者
杨丽勤
李峙玉
檀沛
徐文婷
张忠楠
汪萌芽
YANG Liqin;LI Zhiyu;TAN Pei;XU Wenting;ZHANG Zhongnan;WANG Mengya(Cell Electrophysiology Laboratory,Wuhu 241002,Anhui,China;Rising-Star Group,Wannan Medical College,Wuhu 241002,Anhui,China)
出处
《中国临床药理学与治疗学》
CAS
CSCD
2019年第12期1341-1346,共6页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
国家级大学生创新创业训练计划项目(201710368020)
国家自然科学基金项目(31271155)资助
