摘要
背景:目前国内外学者试图通过改变植入材料的种类和形貌、改良诱导膜厚度、光滑程度等机械化学性能来促进植骨生长。目的:比较大鼠股骨骨缺损处不同表面粗糙程度聚甲基丙烯酸甲酯骨水泥形成的诱导膜在膜内血管化程度和部分成骨因子表达的差异。方法:取48只雄性SD大鼠(购自广州中医药大学实验动物中心)建立大鼠临界尺寸股骨缺损模型,按随机数字表法分为A、B、C、D组,分别在股骨骨缺损处植入表面粗糙度<1.5 m、1.5-2.0 m、5.0-7.0 m、14.0-20.0 m的聚甲基丙烯酸甲酯骨水泥占位器。植入6周大鼠体内诱导膜形成后取出骨水泥周围的诱导膜,苏木精-伊红染色观察诱导膜病理组织形态结构变化,采用Western Blot印迹方法和免疫组织化学染色法对诱导膜中骨形态发生蛋白2、转化生长因子β1、血管内皮生长因子蛋白进行定量和定性分析。实验获得广州中医药大学动物实验伦理委员会批准,批准号:20181101006。结果与结论:①苏木精-伊红染色显示,4种表面粗糙程度不同的骨水泥均可以形成较为规则的诱导膜,4组诱导膜之间血管化程度和细胞的数量大体相似;②Western Blot印迹检测显示,各组诱导膜内骨形态发生蛋2、转化生长因子β1、血管内皮生长因子蛋白平均含量基本相似(P>0.05);③免疫组织化学染色显示,各组诱导膜内骨形态发生蛋2、转化生长因子β1、血管内皮生长因子蛋白阳性表达基本相似(P>0.05);④结果表明,骨水泥表面粗糙程度改变对诱导膜的组织形态结构和骨形态发生蛋2、转化生长因子β1、血管内皮生长因子表达在6周时无明显影响。
BACKGROUND:At present,scholars at home and abroad try to promote the growth of bone graft by changing the types and morphology of implant materials,and improving the thickness and smoothness of induced membrane.OBJECTIVE:To compare the degree of vascularization and the expression of some osteogenic factors in the polymethylmethacrylate bone cement membrane with different surface roughness degrees at different femoral bone defects in rats.METHODS:Forty-eight male Sprague-Dawley rats(purchased from the Experimental Animal Center of Guangzhou University of Chinese Medicine)were used to establish rat models of critical-size femur defect.These rat modes were then randomly divided into groups A,B,C and D.Polymethylmethacrylate bone cement occupiers with surface roughness<1.5μm,1.5-2.0μm,5.0-7.0μm,and 14.0-20.0μm were implanted in the femoral bone defects,respectively.At 6 weeks after implantation of bone cement spacer,the induced membranes around bone cement were removed.The morphological changes of the induced membranes were observed by hematoxylin-eosin staining.Western blotting and immunohistochemical staining were used to detect bone morphogenetic protein 2,transforming growth factor beta 1 and vascular endothelial growth factor protein in the induced membranes for quantitative and qualitative analyses.This study was approved by the Animal Ethics Committee of Guangzhou University of Chinese Medicine,China(approval No.20181101006).RESULTS AND CONCLUSION:Hematoxylin-eosin staining showed that four kinds of bone cement with different surface roughness degrees could form a relatively regular induced membrane.The degree of vascularization and the number of cells in the induced membrane were similar among the four groups.Western blot analysis showed that the average levels of bone morphogenetic protein 2,transforming growth factorβ1 and vascular endothelial growth factor protein were similar among four groups(P>0.05).Immunohistochemical staining revealed that the positive expression of bone morphogenetic protein 2,transforming growth factorβ1 and vascular endothelial growth factor protein was similar among four groups(P>0.05).These findings suggest that the change of surface roughness of bone cement has no significant effect on the histomorphological structure of induced membrane and the expression of bone morphogenetic protein 2,transforming growth factorβ1 and vascular endothelial growth factor in induced membrane at 6 weeks after implantation of bone cement spacer.
作者
李树源
周琦石
李悦
林知毅
周宏亮
申震
胡柽
杨佳宝
Li Shuyuan;Zhou Qishi;Li Yue;Lin Zhiyi;Zhou Hongliang;Shen Zhen;Hu Cheng;Yang Jiabao(The First Clinical Medical College,Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;First Department of Orthopedics,First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;Panyu Hospital of Chinese Medicine,Guangzhou 511400,Guangdong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2020年第10期1502-1507,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金资助项目(81674001)，项目负责人：周琦石~~
关键词
聚甲基丙烯酸甲酯骨水泥
表面形貌
诱导膜
微血管
骨生长因子
骨形态发生蛋2
转化生长因子Β1
血管内皮生长因子
polymethylmethacrylate bone cement
surface morphology
induced membrane
microvessel
bone growth factor
bone morphogenetic protein 2
transforming growth factorβ1
vascular endothelial growth factor
