摘要
目的探究miR-34a在NK/T细胞淋巴瘤组织(ENKTCL)表达水平及其对增殖、凋亡和侵袭的影响。方法选取鼻型NK/T细胞淋巴瘤的石蜡组织样本60例为试验组,另选慢性鼻腔炎患者标本20例作为对照组,采用实时荧光定量PCR(qRT-PCR)检测2组标本miR-34a相对表达量,使用MTT法检测细胞增殖、AnncxinV-FITC/PI双染流式细胞检测凋亡、Transwell小室检测细胞侵袭能力。结果miR-34a在NK/T细胞淋巴瘤组织中较对照组表达显著下调(P<0.05)。miR-34a低水平表达组和miR-34a高水平表达组在总生存率上差异无统计学意义(P=0.256),多因素COX回归分析验证IPI评分>3分是患者预后质量差的重要危险因素。miR-34a高表达组肿瘤细胞增殖能力显著低于阴性对照组和干扰组(P<0.05);miR-34a高表达组肿瘤细胞凋亡率显著高于空白对照组和干扰组(P<0.05);miR-34a高表达组侵袭能力显著低于阴性对照组和空白对照组(P<0.05)。结论miR-34a在NK/T细胞淋巴瘤组织中表达下降,能够抑制肿瘤细胞的生长,促进凋亡并降低肿瘤细胞的侵袭能力,认为miR-34a可能是NK/T细胞淋巴瘤的重要分子作用靶点。
Objective To investigate the expression level of microRNA 34a in the tissue of NK/T cell lymphoma(ENKTCL)and its effects on proliferation,apoptosis and invasion.Methods A total of 60 paraffin tissue samples from patients with nasal type NK/T cell lymphoma were selected as experimental group,and 20 samples of patients with chronic rhinitis were selected as control group.The relative expression levels of microRNA 34a were detected by real time fluorescent quantitative PCR(qRT PCR),the cell proliferation was detected by MTT method,moreover the cell apoptosis was detected by AnncxinV FITC/PI double staining flow cytometry.In addition the cell invasion ability was detected by Transwell chamber.Results The expression levels of miRNA 34a in NK/T cell lymphoma tissue were significantly lower than those in control group(P<0.05).However there was no significant difference in the overall survival rate between the miRNA 34a low level expression group and miRNA 34a high level expression group(P>0.05).The multivariate COX regression analysis showed that IPI score>3 was an important risk factor for poor prognosis.The proliferation ability of tumor cells in miRNA 34a high expression group was significantly lower than that in the negative control group and that in interference group(P<0.05).In addition the apoptosis rate of tumor cells in miRNA 34a high expression group was significantly higher than that in blank control group and that in interference group(P<0.05).Moreover the invasion ability in miRNA 34a high expression group was significantly lower than that in negative control group and that in blank control group(P<0.05).Conclusion The down regulation of miRNA 34a expression in NK/T cell lymphoma can inhibit the growth of tumor cells,promote apoptosis and reduce the invasive ability of tumor cells.It is suggested that miRNA 34a may be an important molecular target of NK/T cell lymphoma.
作者
蒲怀智
文雯
王文辉
PU Huaizhi;WEN Wen;WANG Wenhui(Department of Oncology Affiliated Hospital of Panzhihua College,Sichuan,Panzhihua 617000,China)
出处
《河北医药》
CAS
2019年第22期3465-3468,共4页
Hebei Medical Journal
