摘要
目的:研究华东膜蕨中[口山]酮类化学成分及其细胞毒活性。方法:采用硅胶柱色谱、Sephadex LH-20柱色谱及半制备HPLC色谱等分离纯化,结合化合物的理化性质,通过波谱法鉴定其结构,并采用MTT法测定其细胞毒活性。结果:从华东膜蕨中分离得到9个[口山]酮类化合物,分别鉴定为:trapezifolixanthone(1)、cudraxanthone G(2)、8-deoxygartanin(3)、1,5,8-trihydroxy-3-methoxy-2-(3-methylbut-2-enyl)xanthone(4)、gartanin(5)、3-methoxycheffouxanthon(6)、1,5-dihydroxy-3-methoxy-4-isoprenylxanthone(7)、4-(3′,7′-dimethylocta-2′,6′-dienyl)-1,3,5-trihydroxy-9H-xanthen-9-one(8)、cheffouxanthone(9)。细胞毒活性显示,化合物1、6和7对3种人体癌细胞均有不同程度的抑制作用。结论:以上化合物均为首次从该植物中分离得到,化合物1、6和7对3种人体癌细胞具有抑制活性。
Objective:To investigate the xanthones chemical constituents from Hymenophyllum barbatum and their cytotoxic activities.Methods:The chemical constituents were isolated and purified by column chromatography such as silica gel,Sephadex LH-20 and HPLC,and their structures were identified by spectrum analysis.The cytotoxic activities of the isolated compounds in vitro were studied by MTT method.Results:Nine compounds were isolated and identified as trapezifolixanthone(1),cudraxanthone G(2),8-deoxygartanin(3),1,5,8-trihydroxy-3-methoxy-2-(3-methylbut-2-enyl)xanthone(4),gartanin(5),3-methoxycheffouxanthon(6),1,5-dihydroxy-3-methoxy-4-isoprenylxanthone(7),4-(3′,7′-dimethylocta-2′,6′-dienyl)-1,3,5-trihydroxy-9 H-xanthen-9-one(8),cheffouxanthone(9).Cytotoxic activity results showed that compounds 1,6 and 7 had different degrees of inhibition on the three types of human cancer cells.Conclusion:All compounds are isolated from Hymenophyllum barbatum for the first time.Compounds 1,6 and 7 have better inhibitory activities against three types of human cancer cells.
作者
陈祥云
高映
潘玲玲
卢健
陈圣加
舒积成
CHEN Xiang-yun;GAO Ying;PAN Ling-ling;LU Jian;CHEN Sheng-jia;SHU Ji-cheng(Jiangxi University of Traditional Chinese Medicine/Key Laboratory of Modem Preparation of Traditional Chinese Medicine,Ministry of Education,Nanchang 330004,China)
出处
《中药材》
CAS
北大核心
2019年第3期556-559,共4页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(81760721,81760703)
江西省自然科学基金(20161BAB205219,20142BCB23023)
江西省卫生厅课题(2016A062)
关键词
华东膜蕨
[口山]酮
化学成分
细胞毒活性
Hymenophyllum barbatum(v.d.B.)HK et Bak.
Xanthones
Chemical constituents
Cytotoxic activities
