摘要
目的 :观察人血浆高密度脂蛋白 (HDL)对大鼠内毒素血症的治疗和防护效果。方法 :采用鲎试剂法和放射免疫分析法于 3个时点动态测定对照组、治疗组和防护组大鼠血浆内毒素 (ET)水平和肿瘤坏死因子 (TNF)浓度并观察血压及存活时间。结果 :①输注ET后对照组大鼠血压进行性下降 (P <0 0 1) ;治疗组大鼠输注HDL后其血压虽也降低但下降程度明显弱于对照组 (P <0 0 1) ;防护组大鼠在输注ET后血压无明显下降 (P >0 0 5 )。治疗组及防护组大鼠存活时间均明显长于对照组 (P <0 0 1)。② 3组大鼠血浆ET水平在各时点均无明显变化 (P >0 0 5 )。③治疗组及防护组大鼠血浆TNFα水平于第 3时点均明显降低 (P >0 0 5 )。结论 :人血浆HDL能减轻或抑制内毒素血症大鼠血压下降并明显延长其存活时间 ,对内毒素血症具有良好的治疗和防护作用 ,此作用可能与其抑制TNF释放有关。
AIM: To observe the therapeutic and protective effects of high-density lipoprotein (HDL) of human plasma on rat endotoxemia.METHODS: 26 rats were divided into 3 groups randomly,the treated group received bolus intravenous endotoxin (500 U/kg),followed by a bolus HDL (75 mg/kg) intravenously as their blood pressure (BP) decreased obviously.The control group just received bolus intravenous endotoxin without HDL.The protective group received bolus intravenous HDL before endotoxin challenge.BP,survival time,concentration of tumor necrosis factor(TNF) and levels of endotoxin in plasma were determined using radioimmunoassay and limulus lysate test.RESULTS: ①BP in control and treated groups all decreased obviously (P<0.01),and the extent of BP decrease in control group lowered more than that of treated group P<0.01),while no significant BP decrease was found in protective group (P>0.05).The survival time of treated and preventive rats were prolonged significantly than that of control group (P<0.01).②No obvious changes were found on the levels of endotoxin among the three groups (P>0.05).③On 3rd time point,the concentration of plasma TNFα decreased in treated and protective rats compared with that of control rats (P>0.05). CONCLUSION:HDL of human plasma could attenuate or inhibit the decrease in BP induced by endotoxin and prolong the survival time.These results indicated that HDL has therapeutic and protective effect on rat with endotoxemia.Inhibition of TNF release might be one of mechanisms.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2002年第10期1241-1243,共3页
Chinese Journal of Pathophysiology
基金
四川省卫生厅科研基金资助项目(No.981039)
