摘要
目的测定人精子冷藏在4 ℃无电解质溶液(EF溶液)中是否保持正常受精能力。方法人精子冷藏在EF溶液中,分别测定其活动率、顶体状态和受精指数。结果人精子冷藏在4 ℃EF溶液中1周,其活动率明显高于冷藏在缓冲的人输卵管溶液中[(43.4±7.9)%比(9.5±2.5)%,P<0.01]。虽然冷藏在EF溶液中的人精子顶体状态在重激活前与新鲜精子无明显不同[获能精子为(7.6±1.8)%比(6.4±1.8)%,顶体反应精子为(3.0±1.7)%比(2.4±1.1)%,P>0.05],但是前者在重激活后其获能精子和顶体反应精子的百分率明显增加[获能精子为(16.0±2.3)%比(7.6±1.8)%,顶体反应精子为(9.4±2.1)%比(3.0±1.7)%,P<0.01]。冷藏在EF溶液中精子的穿透率和受精指数与新鲜精子相似(48.1%比50.9%,1.38±0.16比1.29±0.13,P>0.05)。结论人精子冷藏在4 ℃EF溶液中1周,不发生获能和顶体反应,重激活后具有与新鲜精子一样的受精能力。
Objective To assess the fertilizing capacity of human sperm preserved in electrolyte-free (EF) solution at 4 ℃. Methods The motility, acrosome status and fertility index of human sperm were respectively assessed before and after preser-vation in cold EF solution. Results The motility of human sperm so preserved for one week was significantly higher than that of the sperm preserved in modified human tubal fluid (43.4±7.9% vs9.5±2.5%, P<0.01). Although acrosome status of human sperm in EF solution before reinitiation differed little from that of fresh sperm (capacitated sperm: 7.6±1.8% vs6.4±1.8%; acrosome-reacted sperm: 3.0±1.7% vs2.4±1.1%, P>0.05), the percentage of capacitated and acrosome-reacted sperm in the EF solution significantly increased after reinitiation (16.0±2.3% vs7.6±1.8% and 9.4±2.1% vs3.0±1.7% respectively, P<0.01). The penetration rate and fertility index of the sperm in EF solution were comparable with those of fresh sperm (48.1% vs50.9%; 1.38±0.16 vs1.29±0.13, respectively). Conclusions Preservation in cold EF solution does not induce human sperm to undergo capacitation and acrosome reaction, and the sperm so preserved for one week possesses comparable fertilizing capaci-ty as fresh sperm does.
出处
《第一军医大学学报》
CSCD
北大核心
2002年第10期928-930,933,共4页
Journal of First Military Medical University
关键词
精子
无电解质溶液
低温保藏
顶体反应
精子能动性
sperm
electrolyte-free solution
cryopreservation
acrosome reaction
sperm motility
