摘要
目的探讨热休克蛋白(heat shock protein,HSP)70的诱导表达对豚鼠耳蜗听功能的影响。方法将经白噪声预刺激(100 dB SPL,45 min)诱导耳蜗产生HSP70的豚鼠与无预刺激的正常豚鼠同时暴露于强噪声(125 dB SPL,90 min)中,观察强噪声刺激后不同时间的听性脑干反应(ABR)阈值。结果100 dB SPL的白噪声能诱导耳蜗HSP70的表达。强刺激后12 h,两组间ABR阈值无显著差异(P>0.05);强刺激后60 和108 h,预刺激组的ABR阈值均低于无预刺激组(P<0.01)。在预刺激组内,108 h ABR阈值低于60 h ABR阈值(P<0.05),而在无预刺激组内,108和60 h ABR阈值无显著差异(P>0.05)。结论经预刺激诱导耳蜗产生的HSP70对豚鼠耳蜗听功能具有保护作用。
Objective To investigate the effect of evoked heat shock protein 70 (HSP70) expression on the hearing function of the cochlea in guinea pigs. Methods Guinea pigs were divided into pre-exposure group (pre-treated with white noise exposure at 100 dB SPL for 45 min) and none pre-exposure group. Auditory brainstem response (ABR) thresholds were recorded at 12, 60 and 108 h after the animals in both groups were exposed to loud white noise (125 dB SPL, 90 min). Results HSP70 expression was evoked by pre-treatment with white noise (100 dB SPL, 45 min). There was no significant difference of ABR thresholds between the 2 groups (P>0.05) at 12 h after exposure to loud noise, while ABR thresholds became lower in pre-exposure group than in none pre-exposure group (P<0.01) at both 60 and 108 h. In the pre-exposure group, ABR thresholds at 108 h were significantly lower than that measured at 60 h (P<0.05), but which was not the case in none pre-exposure group. Conclusion HSP70 expression induced by pre-exposure to noise protects the hearing function of the cochlea in guinea pigs.
出处
《第一军医大学学报》
CSCD
北大核心
2002年第10期922-924,共3页
Journal of First Military Medical University
关键词
热休克蛋白质类70
耳蜗
诱发电位
听觉
脑干
测听法
诱发反应
噪声
heat shock protein 70
cochlea
evoked potentials, auditory, brainstem
audiometry, evoked response
noise
