摘要
以超滤和蔗糖密度梯度超速离心法浓集和纯化猴艾滋病D型逆转录病毒SRV-1感染的Raji细胞培养上清,制备出含SRV-1结构蛋白的抗原制剂。以含有0.02％NaN_3的碳酸盐缓冲液作为抗原包被液,不加任何封闭剂,待测血清以含有0.2％NaN_3和0.05％Tween-20的PBS稀释,以山羊或兔抗人IgG的辣根过氧化物酶结合物作为酶标抗体,成功地检测出猴血清中抗SRV抗体,并以Western blot最后确诊。
Viral structural protein antigens of simian type D retrovirus ( SRV ) were prepared by ultrafiltration and sucrose gradient ultracentrifugation of supernatants of SRV-1 infected Raji cell culture. Carbonate buffer containing 0.02% NaN3 was used for coating the antigens. Without adding proteinous blocking agents to microplate wells, monkey sera examined were diluted with 0.2% NaN3-0.05% Tween-20 in PBS. Conjugates of horseradish peroxidase and goat or rabbit anti-human IgG were applied to ELISA. Antibodies to SRV in macaque sera were successfully determined by the ELISA and were further confirmed by Western blot.
出处
《病毒学报》
CAS
CSCD
北大核心
1992年第4期354-359,共6页
Chinese Journal of Virology
基金
云南省应用基础研究基金
云南灵长类中心和昆明动物所灵长类生物学联合实验室基金
