摘要
目的 在体内和体外研究SEA再次刺激后Vβ3+ CD4+ 和Vβ3+ CD8+ T细胞亚群的免疫反应 ,探讨CD4+ 和CD8+ T细胞亚群对超抗原SEA反应性的差异。方法 分离小鼠超抗原金黄色葡萄球菌肠毒素A(SEA)应答性Vβ3+ CD4+ 和Vβ3+ CD8+ 两个T细胞亚群 ,检测其对SEA再次刺激的增殖反应及产生IL 2活性。结果 Vβ3+ CD4+ 和Vβ3+ CD8+ T细胞亚群对SEA再次刺激所诱导的增殖反应以及产生IL 2活性的能力有显著差异 (P <0 .0 1)。结论 SEA单次刺激时Vβ3+ CD4+ 和Vβ3+ CD8+ T细胞亚群反应性相似 ,再次刺激时Vβ3+ CD8+ 呈显著低反应性。
Purpose: Examined the immunologic behaviors of (staphylococcal enterotoxin A, SEA) SEA-reactive two distinct T cell populations (Vβ3+ CD4+, Vβ3+ CD8+ T cells) in vivo and in vitro restimulated with SEA. Study the responsiveness of CD4+ and CD8+ T cell subsets reactive with superantigen SEA. Methods: Examined the proliferation and the IL-2 activity of the two purified murine SEA-reactive Vβ3+ CD4+ and Vβ3+ CD8+ T cell subsets that were restimulated with SEA. Results: Vβ3+ CD4+, Vβ3+ CD8+ T cells subsets showed significantly difference in proliferation and IL-2 activity restimulated with SEA (P+ CD4+ and Vβ3+ CD8+ T cell subsets in single SEA stimulation, but the responsiveness of Vβ3+ CD4+ and Vβ3+ CD8+ T cell subsets were changed in SEA restimulation.
出处
《复旦学报（医学版）》
EI
CAS
CSCD
北大核心
2002年第6期437-439,共3页
Fudan University Journal of Medical Sciences
