摘要
目的 探讨 ATP敏感性钾通道 (KATP)开放剂吡那地尔对大鼠心肌缺血 /再灌注时心肌细胞凋亡及 fas基因蛋白表达的调节作用 .方法 4 0只大鼠随机分成 4组 :1假手术组 (仅穿线而不结扎 ,观察 6 .5 h) ;2缺血 /再灌注组(缺血 30 min,再灌注 6 h) ;3吡那地尔组 (缺血前 10 m in静推吡那地尔 0 .2 mg· kg- 1 ,然后缺血 30 min/再灌注 6 h) ;4吡那地尔 +优降糖组 (缺血前 10 min静推吡那地尔 0 .2 m g·kg- 1 +优降糖 3mg·kg- 1 ,然后缺血 30 min/再灌注 6 h) .称质量法计算心肌梗死范围 ,以缺口末端标记法 (TUNEL)检测凋亡细胞 ,以 SABC免疫组化法检测 fas蛋白的表达 .结果 缺血 /再灌注组出现显著的心肌细胞凋亡 ,伴有 fas蛋白表达指数升高 (P<0 .0 1vs假手术组 ) ;吡那地尔可抑制心肌细胞凋亡 ,并显著下调 fas蛋白的表达 (P<0 .0 5 ) ,而优降糖可取消吡那地尔的作用 .结论 细胞凋亡及 fas基因表达改变参与了心肌缺血 /再灌注损伤过程 ,KATP开放剂 (吡那地尔 )可抑制心肌细胞凋亡、下调 fas基因的蛋白表达 .
AIM To study the effects of ATP sensitive potassium channel opener (pinacidil) on apoptosis and fas gene expression in rat cardiomyocytes during myocardial ischemia/reperfusion. METHODS Forty rats were randomly divided into four groups: sham operated group, ischemia/reperfusion group (30 minutes of ischemia followed by six hours of reperfusion), pinacidil group and pinacidil + glybenclamide group. The myocardial cell apoptosis was determined with terminal deoxynucleotidyl transferase mediated dUTP fluorescein nick end labeling (TUNEL) method. The protein expression of fas gene was detected by SABC immunohistochemical staining. RESULTS Myocardial apoptosis index and the level of fas gene expression significantly increased in ischemia/reperfusion group compared with that in the sham operated group ( P <0.01). Pinacidil decreased cardiomyocyte apoptosis and down regulated the protein expression of fas gene ( P <0.05), whereas glybenclamide offset the effects of pinacidil. CONCLUSION Changes of apoptosis ratio and fas gene expression may be involved in the process of myocardial ischemia/reperfusion injury. ATP sensitive potassium channel opener (pinacidil) can inhibit cardiomyocyte apoptosis and down regulate the protein expression of fas gene.
出处
《第四军医大学学报》
北大核心
2002年第22期2067-2070,共4页
Journal of the Fourth Military Medical University
