摘要
目的观察缺血预处理对大鼠移植胰细胞凋亡的影响。方法6只正常大鼠为对照组,18只糖尿病SD大鼠随机分为缺血再灌注组(I/R组,n=6)、供胰缺血预处理组(DIPC组,n=6)和受体双后肢缺血预处理组(RIPC组,n=6),均行单纯胰腺移植(18只正常SD大鼠为供体);检测各组大鼠再灌注前、后血糖;再灌注后2h移植胰组织中超氧化物歧化酶(SOD)和髓过氧化物酶(MPO)含量;用TUNEL法观察移植胰组织细胞凋亡情况。结果与I/R组比较,DIPC组和RIPC组大鼠再灌注后的血糖、MPO活性和移植胰组织细胞调亡指数明显降低,移植胰组织中SOD活性明显增高(均为P<0.01)。结论供胰和受体大鼠双后肢缺血预处理能减轻大鼠移植胰细胞的凋亡现象。
Objective To observe the effect of ischemic preconditioning （IPC） on apoptosis of transplanted pancreas cells in rats. Methods 6 normal SD rats were assigned as control group. 18 steptozozin-induced diabetic SD rats were randomly divided into 3 groups： the I/R group （n= 6, received pancreas transplantation alone）, DIPC group （n= 6, received pancreas transplantation exposed IPC with 5 rain ischemic and 5 rain reperfusion twice） and RIPC group （n=6, received pancreas transplantation exposed IPC with 5 minutes ischemic and 5 minutes reperfusion induced by ligating donors＇ posterior limbs three times before anastomosing vessel）. The blood glucose in serum, superoxide dismutase （SOD） , myeloperoxidase （MPO）, TUNEL cells in graft were monitored. Results After reperfusion, compared with the I/R group, the mean blood glucose levels, MPO levels and apoptotice index of graft reduced, the mean SOD levels of graft heightened in DIPC and RIPC groups significantly （all P〈0.01 ）. Conclusion Ischemic preconditioning induced by graft and ligating donors＇ posterior limbs can reduce apopotosis of transplanted pancreas cells.
出处
《中国康复理论与实践》
CSCD
2005年第8期618-620,共3页
Chinese Journal of Rehabilitation Theory and Practice
关键词
胰腺移植
缺血预处理
缺血再灌注
细胞凋亡
大鼠
pancreas transplantation
ischemic preconditioning
ischemic reperfusion
apoptosis
rat
