摘要
目的 探讨不同浓度甲氨喋呤 (MTX)对培养小鼠骨髓基质细胞 (BMSC)的影响、作用机制及其量效关系。方法 培养的BMSC中加入不同浓度MTX(对照组 :0 μmol/L ;浓度Ⅰ组 :0 0 1μmol/L ;浓度Ⅱ组 :0 1μmol/L ;浓度Ⅲ组 :1 0 μmol/L) ,对BMSC形态学及生长情况、成纤维细胞集落 (CFU F)数目、BMSC层粘附率和造血细胞混合集落生长情况等进行观察比较。结果 浓度Ⅰ组上述指标与对照组无明显差别 ;而浓度组Ⅱ和浓度Ⅲ组梭形成纤维细胞较对照组明显减少 ,生长曲线低平 (P <0 0 5 ,P <0 0 1) ,CFU F计数或粘附率均较正常组显著减少或降低 (P <0 0 1) ,造血细胞集落生长差 ,以浓度Ⅲ组改变最明显。结论 高浓度MTX可导致离体小鼠BMSC形态学改变、生长受抑以及粘附功能和造血支持功能的损伤 。
Objective To explore the effects of different concentrations of Methotrexate (MTX) on murine bone marrow stromal cells (BMSC) in vitro . Methods Cultured BMSCs were added with MTX of 0 μmol/L (control group), 0.01 μmol/L (group Ⅰ), 0.1 μmol/L (group Ⅱ), 1.0 μmol/L (group Ⅲ) respectively, then the morphology and growth of BMSC were observed, the number of colony forming unit fibroblast (CFU F) counted, the adhesive function of BMSC assayed and the growth of colony forming unit mixed hematopoietic cells (CFU Mix) on the BMSC layer observed. Results No remarkable difference was found between the above indexes of concentration Ⅰ group and those of the control. In concentration Ⅱ and Ⅲ groups, however, the number of fibroblast decreased significantly, the growth curve of BMSC lowered, the number of CFU F and the adhesive function reduced significantly and the growth of CFU Mix slowed down. The most remarkable changes were found in concentration Ⅲ group. Conclusion The morphology of BMSC can be changed, the growth of BMSC can be inhibited, and the ability of BMSC to adhere and support hematopoiesis can be damaged by high concentration of MTX. To a certain extent, the damage to BMSC by MTX is dependent upon its concentration.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2002年第11期1273-1275,共3页
Journal of Third Military Medical University
