摘要
利用裸鼠循环系统为实验体系,建立高转移性癌细胞实验转移模型。在此基础上,将体外经白细胞介素2活化的人LAK细胞与高转移性癌细胞同时注射于裸鼠尾静脉内;将未经活化的淋巴细胞与癌细胞一同注射,作为对照,观察LAK细胞抗实验性转移作用。结果表明,每只接受3×10~7 个LAK细胞与1×10~6 个肿瘤细胞的实验组5只小鼠,注射细胞后全部健康存活,无一生长肿瘤。而接受与实验组相同数量的淋巴细胞与肿瘤细胞的对照组5只小鼠,分别于注射后第五周在皮下、脊柱旁及肺脏内出现转移瘤生长。说明LAK细胞不仅在体外具有明显的抗肿瘤作用,在裸鼠内也能够抑制高转移癌系实验性转移的发生。
The blood circulatory system of nude mice has been used to study the anti-experimental metastasis effects of LAK cells activated in vitro with interleukin-2 on the highly metastatic tumor lines moving in blood. On the first day of the test, 3×10~7 LAK cells and 1×10~6 tumor cells were mixed and injected tailveinly to each of the mice in test group (n=5), at the effectors/targets ratio of 30:1; while in the controlled group, each mouse was only injected tailveinly with 1×11~8 tumor cells. All of the mice were injected (ip) with rIL-2 300 cu/gram body weight dayly for seven days. As a result, none of the mice in test group was seen with tumor growth (0/5), eight weeks after injection; and all of the mice in controlled group were fount out with metastatic tumor formation in lungs, lymphonodes, rachis and subskin (5/5). The results indicated that LAK cells could not only exhibit the antitumor effecs in vitro, but also catch and kill metastatic cancer cells in the blood circulation.
关键词
LAK细胞
裸鼠
肿瘤转移
interleukin-2(IL-2),LAK celIs,nude mice,tumor metastasis
