摘要
以α-和β-萘乙酸酯为底物,以固蓝 B 盐和 RR 盐为偶联剂,用聚丙烯酰胺垂直平板电泳技术研究了水稻酯酶同工酶的电泳显色,结果为①少数酶带只能用α-萘乙酸酯显示;绝大多数酶带可用α-或β-萘乙酸酯显示。②用α-和β-萘乙酸酯同时显色时,一些单独用α-或β-萘乙酸酯能显示的酶带的相对染色强度或者降低(甚至酶带完全消失),或者增加。③用固蓝 B 盐作偶联剂与用固蓝 RR 盐相比,某些酶带的相对染色强度增加。因此,各电泳酶带染色强度的相对强弱并不一定反应各酶本身活性的相对高低。
With α-naphthyl acetate and/β-naphthyl acetate as substrates and with Fast Blue B salt and Fast Blue RR salt as coupling agents,the rice esterase isoenzyme bands sepa- rated by the method of thin layer polyacrylamide gel were stained.The results were as fol- lows:①Some bands were stained only by with α-napythyl acetate as a substrate:most bands were stained not only by with a-naphthyl acetate as a substrate,but by with β-naph- thyl acetate as a substrate.②The relative staining strength of some bands shown by with α- or β-naphthyl acetate as a substrate was increased or decreased when stained by with mixed substrates of α-and β-naphthyl acetates.③Stained by with Fast Blue B salt as a coupling agent,the relative staining strength of some bands was increased,compared by with Fast Blue RR as a coupling agent.Therefore,the differnces existed in the relative staining strength of esterase electrophoretical bands as well as in the constitution of the isozymo- gram,when stained by different staining agents.The relative staining strength of the elec- trophoretical bands didn't necessarily represent the relative activities or amount of esterase isoenzymes.
关键词
酯酶同工酶
染色
电泳
水稻
esterase isoenzyme
staining
PAGE
rice
