摘要
目的 研究逆转录病毒介导的HSV tk/GCV系统对人喉癌细胞的体外杀伤作用。方法 构建携带HSV tk基因的逆转录病毒载体 ,并以该重组逆转录病毒感染人喉癌细胞Hep 2。以G4 18筛选的抗性克隆 (命名为Hep 2 /tk) ,用MTT比色法观察GCV在体外对Hep 2 /tk细胞的杀伤作用。结果 Hep 2 /tk细胞与未转染tk基因的Hep 2 /0、Hep 2细胞相比较 ,三者的生长速度无明显差别。Hep 2 /tk细胞对GCV高度敏感 ,即低浓度GCV(0～ 1mg/L)处理Hep 2 /tk细胞 7d,可将其大部分细胞杀死 ,增加GCV的浓度 (>1mg/L)不能显著增强其对Hep 2 /tk细胞的杀伤作用。 结论 人喉癌细胞Hep 2对HSV tk/GCV系统高度敏感 。
Aim To observe the cytotoxicity in vitro of the retrovirus mediated HSV tk/GCV system to human laryngocarcinoma cells Hep 2. Methods The recombinant retroviral vector containing HSV tk gene was constructed and human Hep 2 cells was infected with the recombinant retrovirus. Hep 2/tk cells which expressed stably tk were screened with G418. The cytotoxicity of GCV to Hep 2/tk cells were observed in vitro . Results There was no significant difference in proliferations of Hep 2/tk, Hep 2/0 and Hep 2 cells. Hep 2/tk cells were highly sensitive to GCV, especially at low concentration of GCV. Conclusion Hep 2 cells are highly sensitive to HSV tk/GCV system. These is promise to provide an effective measure for treatment of laryngocarcinoma in clinic.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2002年第6期582-585,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助No .3 9970 788
